Department of Microbiology, University of Karachi, Karachi 75270, Pakistan.
Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
Int J Biol Macromol. 2019 Nov 1;140:1064-1072. doi: 10.1016/j.ijbiomac.2019.08.206. Epub 2019 Aug 24.
The present study was aimed to evaluate the Methyltrioctylammonium Chloride (IL) and Sodium hydroxide effect on sugarcane bagasse (SB) structure and its subsequent utilization to produce cellulase from a thermophilic bacterium Bacillus aestuarii UE25. The strain was isolated from a crocodile pond of Manghopir, Karachi. Ten different factors affecting IL pretreatment of SB and cellulase production by UE25 were evaluated by Plackett-Burman design and three significant factors were optimized by employing Box Behnken design. Under optimum conditions, the strain produced 118.4 IU mL of EG and 75.01 IU mL of BGL that corroborated well with the predicted values by the model. Scanning electron microscopy, gravimetric analysis, Fourier transform infrared spectroscopy and NMR of SB revealed removal of lignin, decrease in cellulose content and structural changes in the SB after pretreatment and fermentation. The data provide prospects of utilizing this IL in comparison to imidazolium based IL for pretreatment of biomass.
本研究旨在评估甲基三辛基氯化铵（IL）和氢氧化钠对甘蔗渣（SB）结构的影响，以及随后利用嗜热细菌巴氏芽孢杆菌 UE25 从甘蔗渣中生产纤维素酶。该菌株是从卡拉奇曼戈皮尔的鳄鱼池塘中分离出来的。通过 Plackett-Burman 设计评估了 10 种不同因素对 SB 的 IL 预处理和 UE25 产生纤维素酶的影响，并通过 Box-Behnken 设计对 3 个显著因素进行了优化。在最佳条件下，该菌株产生了 118.4IU/mL 的 EG 和 75.01IU/mL 的 BGL，与模型预测值吻合较好。SB 的扫描电子显微镜、重量分析、傅里叶变换红外光谱和 NMR 显示，预处理和发酵后木质素去除、纤维素含量降低以及 SB 的结构发生变化。与基于咪唑的 IL 相比，该数据为 IL 在生物质预处理中的应用提供了前景。
