Department of Biosciences, Faculty of Life Sciences, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology (SZABIST), Karachi, 75600, Pakistan.
Department of Microbiology, University of Karachi, Karachi, 75600, Pakistan.
Antonie Van Leeuwenhoek. 2023 Jul;116(7):653-665. doi: 10.1007/s10482-023-01835-y. Epub 2023 May 4.
Genetic and enzymatic potential of Neobacillus sedimentimangrovi has not been assembled to date. Here, we report a high-quality genome assembly of thermophilic bacterium Neobacillus sedimentimangrovi UE25 using Illumina Hi-seq 2500. The strain was isolated from a crocodile pond Manghopir, Karachi, Pakistan. QUAST quality parameters showed 37.75% GC content and exhibited the genome into 110 contigs, with a total size of 3,230,777 bases. Genome of N. sedimentimangrovi UE25 harbors phage mediated DNA through horizontal gene exchange from the phages, symbiotic and pathogenic bacteria. Most of the phage genome encodes for hypothetical proteins, protease, and phage assembly proteins. Gene clusters encoding the intrinsic resistance to glycopeptides, isoniazid, rifamycin, elfamycin, macrolide, aminoglycosides, tetracycline and fluoroquinolone were identified into the genome. Since, the strain has been reported for the production of many industrially important thermostable enzymes, therefore, the genomic data of thermostable enzymes might be helpful to employ this species in commercial sectors. Probing genes of multiple thermostable glycoside hydrolase enzymes especially xylanases of N. sedimentimangrovi UE25 showed genetic diversity among the genes and confer the industrial importance of this microorganism. Furthermore, the genome of N. sedimentimangrovi will greatly improve our understanding of its genetics and evolution.
到目前为止，还没有对 Neobacillus sedimentimangrovi 的遗传和酶学潜力进行组装。在这里，我们使用 Illumina Hi-seq 2500 报道了嗜热细菌 Neobacillus sedimentimangrovi UE25 的高质量基因组组装。该菌株是从巴基斯坦卡拉奇 Manghopir 的鳄鱼池塘中分离出来的。QUAST 质量参数显示其 GC 含量为 37.75%，并将基因组分为 110 个连续序列，总大小为 3230777 个碱基。N. sedimentimangrovi UE25 的基因组通过噬菌体介导的水平基因交换从噬菌体、共生菌和病原菌中携带噬菌体 DNA。大多数噬菌体基因组编码假定蛋白、蛋白酶和噬菌体组装蛋白。鉴定出的基因簇可编码对糖肽、异烟肼、利福霉素、elfamycin、大环内酯、氨基糖苷类、四环素和氟喹诺酮的固有抗性。由于该菌株已被报道可生产许多工业上重要的耐热酶，因此耐热酶的基因组数据可能有助于该物种在商业领域的应用。对 N. sedimentimangrovi UE25 的多种耐热糖苷水解酶基因（特别是木聚糖酶）的基因进行探测，显示出基因之间的遗传多样性，并赋予了该微生物的工业重要性。此外，Neobacillus sedimentimangrovi 的基因组将极大地提高我们对其遗传学和进化的理解。
