Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Photodiagnosis Photodyn Ther. 2019 Dec;28:98-101. doi: 10.1016/j.pdpdt.2019.08.028. Epub 2019 Aug 24.
The aim of the present randomized clinical study was to compare the efficacies of photodynamic inactivation (PDI) to nystatin (NST) in terms of prevalence of Candida species in cases with denture stomatitis (DS).
Thirty-six patients were randomly divided into two groups; 18 in PDI and 18 in NST. Irradiation was carried out by using the GaA1As diode laser with wavelength, mode of transmission, laser output and energy density were standardized at 660 nm, continuous mode, 100 mW power and 28 J/cm2 respectively. The PDI was applied twice a week, with an interval of at least 48 h among the sessions during four weeks. Topical nystatin oral suspension 100,000 IU was used four times daily for 15 days. The existence of Candida spp. was confirmed by employing the microbiological culture technique. Candida colony counts from the palates and dentures surfaces, quantified as colony forming unit (CFU)/mL, measured at baseline, at the end of treatments (day 15), and at follow-up (days 30 and 60) and the prevalence of Candida spp. were identified in the two groups of treatments.
The overall CFU/mL values were higher in the dentures of the patients of both the groups than those from the palates. During all time periods of the study, the CFU/mL values obtained from both NST and PDI groups showed no significant differences. For dentures and palates, a significant reduction in mean CFU/mL values was observed on day 15 compared with baseline (day 0) in both NST and PDI groups. It can be seen that the effect size of treatments was large for the palates of patients in the NST group (1.79) and moderate for the palates of patients in the PDI group (0.63). On the other hand, the effect size was very large for the dentures for both groups (NST group = 3.01; PDI group = 1.58). C. albicans was the most common species on both dentures and palates of patients throughout the study period followed by C. tropicalis and C. glabrata.
Out of all the Candida spp., C. albicans showed the highest prevalence among all species. In addition, PDI was equally effective as nystatin for the treatment of DS.
本随机临床研究旨在比较光动力疗法（PDI）与制霉菌素（NST）治疗义齿性口炎（DS）时，在念珠菌种类流行率方面的疗效。
将 36 名患者随机分为两组，18 名患者接受 PDI，18 名患者接受 NST。使用 GaA1As 二极管激光进行照射，波长、传输模式、激光输出和能量密度分别标准化为 660nm、连续模式、100mW 功率和 28J/cm2。PDI 每周应用两次，两次治疗之间至少间隔 48 小时，持续四周。每天使用 10 万 IU 制霉菌素口腔混悬液 4 次，共 15 天。采用微生物培养技术证实念珠菌 spp.的存在。在基线、治疗结束时（第 15 天）和随访时（第 30 天和第 60 天），测量腭部和义齿表面的念珠菌菌落数，以菌落形成单位（CFU）/mL 表示，并在两组治疗中确定念珠菌 spp.的流行率。
两组患者的义齿 CFU/mL 值均高于腭部。在研究的所有时间段，NST 和 PDI 组获得的 CFU/mL 值均无显著差异。对于义齿和腭部，与基线（第 0 天）相比，NST 和 PDI 组在第 15 天的平均 CFU/mL 值均显著降低。可以看出，NST 组患者腭部的治疗效果显著（效应量为 1.79），PDI 组患者腭部的治疗效果中等（效应量为 0.63）。另一方面，两组义齿的治疗效果非常显著（NST 组为 3.01；PDI 组为 1.58）。在整个研究期间，C. albicans 是患者义齿和腭部最常见的菌种，其次是 C. tropicalis 和 C. glabrata。
在所研究的所有念珠菌中，C. albicans 在所有菌种中的流行率最高。此外，PDI 与制霉菌素治疗 DS 的疗效相当。
