State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; National Engineering Laboratory for Sustainable Sludge Management & Resourcelization Technology, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; National Engineering Laboratory for Sustainable Sludge Management & Resourcelization Technology, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2019 Dec;293:122004. doi: 10.1016/j.biortech.2019.122004. Epub 2019 Aug 13.
The integrated effects of temperature and COD/N ratio on performance, biofilm characteristics and microbial community in up-flow anaerobic filter-biological aerated filters (UAF-BAFs) were investigated. Results indicated that the UAF-BAF system could achieve excellent COD, NH-N and TN removal, in which effluent quality well met the Class 1A standard. Biofilm physicochemical characteristics showed that the biomass, biofilm thickness and extracellular polymeric substance (EPS) content in the UAF-BAFs reduced with the decrease in COD/N ratio, but were enhanced under low temperature. The biofilm structure characterized by CLSM in the UAF-BAFs significantly shifted, which was closely correlated with operational conditions. Sequencing analysis revealed that Proteobacteria, Epsilonbacteraeota, Bacteroidetes and Firmicutes were dominant in the UAFs and the abundance of ammonium oxidizing bacteria (AOB) was responsible for nitrification performance in the BAFs. Functions analysis indicated that amino acid metabolism, carbohydrate metabolism, energy metabolism and lipid metabolism were clearly regulated by parameters changes.
研究了温度和 COD/N 比对上流式厌氧滤池-曝气生物滤池（UAF-BAF）性能、生物膜特性和微生物群落的综合影响。结果表明，UAF-BAF 系统可以实现优异的 COD、NH4-N 和 TN 去除效果，出水水质完全符合 1A 类标准。生物膜理化特性表明，随着 COD/N 比的降低，UAF-BAF 中的生物量、生物膜厚度和胞外聚合物（EPS）含量减少，但在低温下会增强。UAF-BAF 中 CLSM 表征的生物膜结构发生了显著变化，这与操作条件密切相关。测序分析表明，变形菌门、Epsilonbacteraeota 门、拟杆菌门和厚壁菌门是 UAF 中的优势菌门，氨氧化菌（AOB）的丰度是 BAF 硝化性能的关键。功能分析表明，参数变化明显调控了氨基酸代谢、碳水化合物代谢、能量代谢和脂质代谢等功能。
