Gil-Loyzaga P, Pujol R
INSERM-U.254, Hôpital St. Charles, Montpellier, France.
Int J Dev Neurosci. 1988;6(2):155-60. doi: 10.1016/0736-5748(88)90040-8.
The immunoreactivity to SY38 (anti-synaptophysin antibody) was investigated in rat and guinea-pig cochleas during development. In rat pups SY38 reactivity first appeared in the inner spiral bundle (below inner hair cells) at postnatal day 3. Later on (days 10 and 15) the basal pole of outer hair cells (OHCs) was also reactive. In fetal guinea-pigs, the inner spiral bundle was reactive on day 45 of gestation, while the reactivity occurred below OHCs on day 62 of gestation. A preliminary electron microscopic finding (from a guinea-pig 62 days of gestation) indicated that SY38 immunoreactivity is localized within varicosities of efferent (olivo-cochlear) endings. Synaptophysin is thus present in the cochlea at the level where the two efferent systems terminate. Moreover, the occurrence of SY38 immunoreactivity, first at the ISB then at the OHC levels, is in accordance with the observation that the maturation of lateral efferents precedes that of medial efferents.
在发育过程中，研究了大鼠和豚鼠耳蜗对SY38（抗突触素抗体）的免疫反应性。在新生大鼠中，SY38反应性在出生后第3天首次出现在内螺旋束（在内毛细胞下方）。随后（第10天和第15天），外毛细胞（OHC）的基部也出现反应性。在豚鼠胎儿中，内螺旋束在妊娠第45天出现反应性，而在妊娠第62天，反应性出现在OHC下方。初步的电子显微镜观察结果（来自妊娠62天的豚鼠）表明，SY38免疫反应性定位于传出（橄榄耳蜗）末梢的膨体中。因此，在两个传出系统终止的水平，耳蜗中存在突触素。此外，SY38免疫反应性首先出现在内螺旋束水平，然后出现在OHC水平，这与外侧传出纤维的成熟先于内侧传出纤维的观察结果一致。
