Fan Peixin, Nelson Corwin D, Driver J Danny, Elzo Mauricio A, Jeong Kwangcheol Casey
Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Front Microbiol. 2019 Aug 13;10:1846. doi: 10.3389/fmicb.2019.01846. eCollection 2019.
Antibiotics have been widely used in livestock to treat and prevent bacterial diseases. However, use of antibiotics has led to the emergence of antibiotic resistant microorganisms (ARMs) in food animals. Due to the decreased efficacy of antibiotics, alternatives to antibiotics that can reduce infectious diseases in food animals to enhance animal health and growth performance are urgently required. Here, we show that animal genetics is associated with the hindgut microbiome, which is related to fat deposition and beta-lactam resistance in the gastrointestinal tract. We investigated the hindgut microbiota structure in 95 postweaning heifers belonging to the unique multibreed Angus-Brahman herd with breed composition ranging from 100% Angus to 100% Brahman. The hindgut microbial composition of postweaning heifers differed among breed groups. The mucin-degrading bacterium known for promoting energy expenditure was enriched in Brahman calves that contained less intramuscular fat content, while butyrate-producing bacterium was linearly positively correlated with Angus proportion. Moreover, the higher relative abundance of beta-lactam resistant genes including ampC gene and arcA gene was associated with the greater Brahman proportion. As the first study aimed at understanding changes in hindgut microbiota among beef cattle with linear gradient of breed composition and its association with marbling in meat, our results suggest that the effects of animal genetics on the gut microbiota structure is associated with fat deposition and potentially a factor affecting the gut antimicrobial resistance.
抗生素已被广泛用于家畜治疗和预防细菌性疾病。然而，抗生素的使用导致了食用动物中出现抗生素抗性微生物（ARMs）。由于抗生素疗效下降，迫切需要能够减少食用动物传染病以促进动物健康和生长性能的抗生素替代品。在此，我们表明动物遗传学与后肠微生物群有关，而后肠微生物群与胃肠道中的脂肪沉积和β-内酰胺抗性有关。我们调查了95头断奶后小母牛的后肠微生物群结构，这些小母牛属于独特的多品种安格斯-婆罗门牛群，品种组成从100%安格斯到100%婆罗门。断奶后小母牛的后肠微生物组成在品种组之间存在差异。以促进能量消耗而闻名的粘蛋白降解细菌在肌肉内脂肪含量较低的婆罗门犊牛中富集，而产丁酸盐细菌与安格斯比例呈线性正相关。此外，包括ampC基因和arcA基因在内的β-内酰胺抗性基因的相对丰度较高与婆罗门比例较高有关。作为第一项旨在了解品种组成呈线性梯度的肉牛后肠微生物群变化及其与肉中大理石花纹相关性的研究，我们的结果表明动物遗传学对肠道微生物群结构的影响与脂肪沉积有关，并且可能是影响肠道抗菌抗性的一个因素。
