Elliyanti Aisyah, Putra Andani Eka, Sribudiani Yunia, Noormartany Noormartany, Masjhur Johan S, Achmad Tri Hanggono, Dachriyanus Dachriyanus
Faculty of Medicine, Universitas Andalas, Padang, Indonesia.
Open Access Maced J Med Sci. 2019 Jul 14;7(13):2088-2092. doi: 10.3889/oamjms.2019.620. eCollection 2019 Jul 15.
This study aims to investigate the effect of ATP, EGF and combination of those two to the Natrium Iodide Symporter (NIS) expression in MCF7, SKBR3 and HaCaT cell lines.
MCF7, SKBR3 and HaCaT cell lines were treated with ATP, EGF and combination of those two for 6, 12 and 24 hours. The expression of NIS mRNA was measured through quantitative-reverse transcription-polymerase chain reaction (qRT-PCR). The NIS protein expression was confirmed by immunocytofluorescence.
NIS mRNA was expressed in SKBR3 and HaCaT cell lines but not in MCF7. The levels of NIS mRNA expression, after treatment by epidermal growth factor (EGF), adenosine Tri-Phosphate (ATP) or the combination of both for 6 and 12 hours were not significantly different from those of untreated cells. However, the treatment by a combination of ATP and EGF for 24 hours increases the level of NIS mRNA expression by 1.6 fold higher than that of the untreated cells (1.6241 ± 0.3, p < 0.05) and protein NIS expression increase significantly by the treatment than untreated cells (P < 0.05).
The level of NIS expression varies among the different subtypes of breast cancer cell lines. MCF7 cell line is representing the luminal A subtype of breast cancer does not express NIS. Only SKBR3 cell line express NIS and this subtype might be suitable to receive radioiodine therapy as those cells expressing NIS. A combination treatment of EGF and ATP increases the expression of NIS mRNA and protein at the membrane in SKBR3 cells.
本研究旨在探讨三磷酸腺苷（ATP）、表皮生长因子（EGF）及其二者组合对MCF7、SKBR3和HaCaT细胞系中碘化钠转运体（NIS）表达的影响。
用ATP、EGF及其二者组合处理MCF7、SKBR3和HaCaT细胞系6、12和24小时。通过定量逆转录聚合酶链反应（qRT-PCR）检测NIS mRNA的表达。通过免疫细胞荧光法确认NIS蛋白的表达。
NIS mRNA在SKBR3和HaCaT细胞系中表达，但在MCF7中不表达。用表皮生长因子（EGF）、三磷酸腺苷（ATP）或二者组合处理6和12小时后，NIS mRNA表达水平与未处理细胞相比无显著差异。然而，ATP和EGF组合处理24小时使NIS mRNA表达水平比未处理细胞提高了1.6倍（1.6241±0.3，p<0.05），且处理后NIS蛋白表达比未处理细胞显著增加（P<0.05）。
NIS表达水平在不同亚型的乳腺癌细胞系中有所不同。代表管腔A型乳腺癌的MCF7细胞系不表达NIS。只有SKBR3细胞系表达NIS，该亚型可能适合接受放射性碘治疗，因为这些细胞表达NIS。EGF和ATP联合处理可增加SKBR3细胞中NIS mRNA的表达及细胞膜上NIS蛋白的表达。
