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通过聚乙二醇化以及包封于海藻酸钠-壳聚糖-聚乙二醇中来实现免疫保护的胰岛正常分泌胰岛素。

Normal Insulin Secretion from Immune-Protected Islets of Langerhans by PEGylation and Encapsulation in the Alginate-Chitosan-PEG.

作者信息

Najafikhah Nahid, Hashemi-Najafabadi Sameereh, Zahediasl Saleh, Nabavimanesh Mohammad Mahdi, Farrokhfall Khadijeh

机构信息

Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.

Endocrine Research Center, Research Institute of Endocrine Sciences, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Biotechnol. 2018 Dec 12;16(4):e1669. doi: 10.21859/ijb.1669. eCollection 2018 Dec.

DOI:10.21859/ijb.1669
PMID:31457032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6697838/
Abstract

BACKGROUND

Pancreatic islet transplantation is one of the most promising strategies for treating patients with type I diabetes mellitus.

OBJECTIVE

We aimed to assess the immunoisolation properties of the multilayer encapsulated islets using alginate-chitosan-PEG for immunoprotection and insulin secretion from the encapsulated islets induced under different glucose concentrations .

MATERIALS AND METHODS

In this study, the islets were isolated from Wistar rats. The biological function (insulin secretion) of the immunoisolated islets following to PEGylation and encapsulation in the alginate-chitosan-PEG, separately, in addition to their immuno-protection in a co-culturing with the lymphocytes isolated from the male C57BL/6 mice were investigated, respectively.

RESULTS

Alginate-chitosan-PEG decreased IL-2 secretion from the lymphocytes co-cultured with islets. Also, insulin secretion from the encapsulated and PEGylated groups was stimulated by glucose (i.e., 5.6 and 16.7 mM of glucose, respectively); showed insulin secretion similar to the naked islets, without coating, after 30 and 60 min of incubation.

CONCLUSION

In conclusion, encapsulation and PEGylation have no negative effect on the insulin secretion and glucose sensitivity of the islets for all of the groups. Also, encapsulation decreased IL-2 secretion from the lymphocytes.

摘要

背景

胰岛移植是治疗I型糖尿病患者最有前景的策略之一。

目的

我们旨在评估使用海藻酸盐-壳聚糖-聚乙二醇（alginate-chitosan-PEG）的多层封装胰岛的免疫隔离特性，以实现免疫保护以及在不同葡萄糖浓度下诱导封装胰岛分泌胰岛素。

材料与方法

在本研究中，胰岛取自Wistar大鼠。分别研究了胰岛在聚乙二醇化并封装于海藻酸盐-壳聚糖-聚乙二醇中后其免疫隔离后的生物学功能（胰岛素分泌），以及与从雄性C57BL/6小鼠分离的淋巴细胞共培养时的免疫保护作用。

结果

海藻酸盐-壳聚糖-聚乙二醇减少了与胰岛共培养的淋巴细胞分泌白细胞介素-2。此外，封装组和聚乙二醇化组的胰岛素分泌受到葡萄糖刺激（即分别为5.6和16.7 mM葡萄糖）；孵育30和60分钟后，其胰岛素分泌与未包被的裸胰岛相似。

结论

总之，封装和聚乙二醇化对所有组的胰岛胰岛素分泌和葡萄糖敏感性均无负面影响。此外，封装减少了淋巴细胞分泌白细胞介素-2。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/13c2be5bb87a/ijb-2018-04-e1669-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/d74609ab14fc/ijb-2018-04-e1669-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/8fd503f18668/ijb-2018-04-e1669-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/e8d0a8cb65b3/ijb-2018-04-e1669-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/13c2be5bb87a/ijb-2018-04-e1669-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/d74609ab14fc/ijb-2018-04-e1669-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/8fd503f18668/ijb-2018-04-e1669-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/e8d0a8cb65b3/ijb-2018-04-e1669-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7bd4/6697838/13c2be5bb87a/ijb-2018-04-e1669-g004.jpg

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