Nowruzi Bahareh, Wahlsten Matti, Jokela Jouni
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Microbiology, Department of Food and Environmental Sciences, University of Helsinki, Finland.
Iran J Biotechnol. 2019 Apr 20;17(2):e1853. doi: 10.21859/ijb.1853. eCollection 2019 Apr.
Cyanobacteria have a worldwide distribution in the terrestrial habitats, occurring predominantly on the surface of the soils, stones, rocks, and trees, practically in moist, neutral or alkaline aeries. The unique natural and bioactive compounds from cyanobacteria with various biological activities and an extensive range of chemical classes have a significant capability for expansion of the pharmaceuticals and other biomedical purposes.
Regardless of the progresses in our knowledge on cyanobacteria, however, cyanobacteria are still viewed as an unexplored source of potential drugs. In this study presence of bioactive compounds among the cyanobacteria culture collection of Iran, where a wide variety of strains can be found, was investigated.
We explored one strain isolated from rice fields in Golestan province of northern Iran for searching for novel products. The chemical construction of the new bioactive compound was clarified by application of liquid chromatography-mass spectrometer (LC-MS) and Marfey's analysis of the degradation products.
We found a novel peptide aldehyde compound from a hydrophilic extract of the Bahar_M, which is composed of the three subunits, 2-hydroxy-4-(4-hydroxyphenyl) butanoic acid (Hhpba), L-Ile, and L-argininal. According to the structural information, we predicted that the novel peptide-aldehyde compound probably to be trypsin inhibitors.
Results demonstrated that terrestrial cyanobacteria are a promissing resource of bioactive natural products.
蓝藻在全球陆地生境中均有分布，主要出现在土壤、石头、岩石和树木表面，实际上多见于潮湿、中性或碱性环境。蓝藻中独特的天然生物活性化合物具有多种生物活性和广泛的化学类别，在制药和其他生物医学领域具有显著的拓展潜力。
尽管我们对蓝藻的认识已有进展，但蓝藻仍被视为潜在药物的未开发来源。本研究对伊朗蓝藻培养物收集库中生物活性化合物的存在情况进行了调查，该收集库中有多种菌株。
我们从伊朗北部戈勒斯坦省稻田分离出一株菌株，以寻找新产物。通过液相色谱 - 质谱联用仪（LC-MS）和对降解产物的马尔菲分析来阐明新生物活性化合物的化学结构。
我们从Bahar_M的亲水性提取物中发现了一种新型肽醛化合物，它由三个亚基组成，即2-羟基-4-(4-羟基苯基)丁酸（Hhpba）、L-异亮氨酸和L-精氨醛。根据结构信息，我们预测这种新型肽醛化合物可能是胰蛋白酶抑制剂。
结果表明陆地蓝藻是生物活性天然产物的一个有前景的资源。
