Nobusawa Kazuyuki, Okamoto Naofumi, Chong Karen Siew Ling, Lin Xi, Iwahori Kenji, Yamashita Ichiro
Graduate School of Engineering, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
ACS Omega. 2017 Apr 12;2(4):1424-1430. doi: 10.1021/acsomega.6b00550. eCollection 2017 Apr 30.
A new method for producing a dispersed gold nanoparticle (Au NP) array to anchor probe DNAs onto a DNA-sensing electrode has been developed. A homogenous gold sulfide (AuS) core (precursor of Au NP) was biomineralized in the cavity of a mutant apoferritin (K98E) with enhanced negative outer-surface charges. We employed a slow chemical reaction system utilizing a stable cationic gold complex. K98E could attract the gold complex, and AuS NPs were synthesized. K98E enabled dispersed placement of the synthesized AuS core onto a cationic 3-aminopropyltriethoxysilane (APTES) layer on a substrate. UV-ozone treatment eliminated the protein shells and APTES layer. X-ray photoelectron spectroscopy confirmed that the AuS core was reduced to Au NPs under the same treatment. Atomic force microscopy (AFM) clearly showed that the combination of apoferritin versatility, chemical system design, and UV-ozone treatment successfully produced a dispersed Au NP array on the substrate.
一种用于制备分散金纳米颗粒（Au NP）阵列以将探针DNA固定到DNA传感电极上的新方法已被开发出来。在具有增强的负外表面电荷的突变脱铁铁蛋白（K98E）的腔内生物矿化形成了均匀的硫化金（AuS）核（Au NP的前体）。我们采用了一种利用稳定阳离子金络合物的缓慢化学反应体系。K98E可以吸引金络合物，并合成了AuS纳米颗粒。K98E能够将合成的AuS核分散放置在基底上的阳离子3-氨丙基三乙氧基硅烷（APTES）层上。紫外臭氧处理去除了蛋白质外壳和APTES层。X射线光电子能谱证实，在相同处理下AuS核被还原为Au纳米颗粒。原子力显微镜（AFM）清楚地表明，脱铁铁蛋白的多功能性、化学体系设计和紫外臭氧处理的结合成功地在基底上制备了分散的Au NP阵列。
