Hacohen Noa, Ip Candice J X, Gordon Reuven
Faculty of Engineering, Department of Electrical and Computer Engineering, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
ACS Omega. 2018 May 16;3(5):5266-5272. doi: 10.1021/acsomega.8b00651. eCollection 2018 May 31.
We use a double nanohole optical tweezer to analyze the protein composition of egg white through analysis of many individual protein trapping events. The proteins are grouped by mass based on two metrics: standard deviation of the trapping laser intensity fluctuations from the protein diffusion and the time constant of these fluctuations coming from the autocorrelation. Quantitative analysis is demonstrated for artificial samples, and then, the approach is applied to real egg white. The composition found from real egg white corresponds well to past reports using gel electrophoresis. This approach differs from past works by allowing for individual protein analysis in heterogeneous solutions without the need for denaturing, labeling, or tethering.
我们使用双纳米孔光镊，通过分析多个单个蛋白质捕获事件来分析蛋清的蛋白质组成。基于两个指标按质量对蛋白质进行分组：蛋白质扩散引起的捕获激光强度波动的标准偏差以及来自自相关的这些波动的时间常数。对人工样品进行了定量分析，然后将该方法应用于实际蛋清。从实际蛋清中发现的组成与过去使用凝胶电泳的报告结果非常吻合。该方法与过去的工作不同，它允许在异质溶液中进行单个蛋白质分析，而无需变性、标记或系留。
