The effects of zinc (Zn2+) and ethylenediaminetetra-acetic acid (EDTA) on tissue cadmium (Cd2+) were investigated in the smooth muscles of rabbit trachea, taenia coli, aorta and guinea-pig taenia coli. 2. After an Cd uptake in high-K2+ solution, samples were rinsed with cold high-K2+, cold Zn2+ or cold EDTA solution without Cd2+ and the results were quantitatively different from each other. 3. Concentration-response curves were obtained with each treatment of cold solution. 4. It is suggested that tissue Cd2+ can be divided into 3 fractions in the surface membrane and there is another fraction in cellular space.