Banfalvi Z, Nieuwkoop A, Schell M, Besl L, Stacey G
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Mol Gen Genet. 1988 Nov;214(3):420-4. doi: 10.1007/BF00330475.
The best inducers of nod::lacZ translational fusions in Bradyrhizobium japonicum are isoflavones, primarily genistein and daidzein. Upstream of the nodABC genes in B. japonicum is a novel gene, nodY, which is coregulated with nodABC. Measurements of the activity of lacZ fusions to the nodD gene of B. japonicum show that this gene is inducible by soybean seed extract and selected flavonoid chemicals. The induction of the nodY ABC and nodD operons appears to require a functional nodD gene, indicating that the nodD gene product controls its own synthesis as well as other nod genes.
在日本慢生根瘤菌中，nod::lacZ翻译融合的最佳诱导剂是异黄酮，主要是染料木黄酮和大豆苷元。日本慢生根瘤菌中nodABC基因的上游是一个新基因nodY，它与nodABC共同调控。对日本慢生根瘤菌nodD基因的lacZ融合活性的测量表明，该基因可被大豆种子提取物和选定的类黄酮化学物质诱导。nodY ABC和nodD操纵子的诱导似乎需要功能性的nodD基因，这表明nodD基因产物控制其自身的合成以及其他nod基因。
