用于分离翻译型乳糖操纵子基因融合体的改良质粒载体。
Improved plasmid vectors for the isolation of translational lac gene fusions.
作者信息
Minton N P
出版信息
Gene. 1984 Nov;31(1-3):269-73. doi: 10.1016/0378-1119(84)90220-8.
PMID:6098531
Abstract
The beta-galactosidase fusion vector pMC1403 has been modified to include the unique cloning sites EcoRI, SmaI, BamHI, SalI, AccI, PstI and HindIII. The new vectors (pNM480, pNM481 and pNM482) allow the fusion of genes to beta-galactosidase in all three translational reading frames, and exhibit an increased sensitivity of promoter detection due to a higher copy number.
摘要
β-半乳糖苷酶融合载体pMC1403已被改造，以包含独特的克隆位点EcoRI、SmaI、BamHI、SalI、AccI、PstI和HindIII。新载体（pNM480、pNM481和pNM482）允许基因在所有三个翻译阅读框中与β-半乳糖苷酶融合，并且由于更高的拷贝数而表现出更高的启动子检测灵敏度。
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