Chen Lihua, Lei Zhongchun, Luo Xiaofang, Wang Dongmei, Li Li, Li An
School of Chemical Engineering and Center of Experiment, Northwest Minzu University, Lanzhou 730030, P. R China.
Department of Chemical Engineering, College of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, P. R. China.
ACS Omega. 2019 Jun 24;4(6):10921-10928. doi: 10.1021/acsomega.9b00906. eCollection 2019 Jun 30.
We report a simple and "green" method for the fabrication of polymer-modified straw-supported oil degradation bacteria (PMS-ODB) for biological degradation of total petroleum hydrocarbons (TPHs) in water. The modification of straw was achieved by in situ copolymerization of styrene and butyl methacrylate using methylene-bis-acrylamide as a cross-linker in an aqueous solution containing straw powders. Compared with the control group (ODB loaded on untreated straw), the results obtained from the experimental group show that the polymer-modified straw is beneficial to the growth of microorganisms. As a result, the degradation rate of TPHs reaches 90.12%, which is 50.54 and 7.08% higher than that of the blank group (ODB only) and the control group, respectively. A study on the transformation characteristics of PMS-ODB shows that the degradation rate of alkanes with low, medium, and high carbon number is higher than 90%. (∑C)/(∑C) (the mass ratio of normal alkanes of high carbon/low carbon), (pr)/(ph) (the ratio of pristane/phytane), and OEP (the mass ratio of normal alkanes of odd carbon/even carbon) for TPHs in the experimental group were measured to be 0.6186, 0.7248, and 1.4356, respectively, all of which are the largest value among the blank group, control group, and experimental group. These findings indicate that compared with the control group, the modification of straw could enhance the comprehensive biological degradation performance for TPHs, even those highly stable organics, such as carbon -alkanes and isoprenoid hydrocarbon, which may open a new possibility for degradation of oils or toxic organics in an enhanced biological manner.
我们报道了一种简单且“绿色”的方法来制备聚合物改性秸秆负载的石油降解细菌（PMS - ODB），用于生物降解水中的总石油烃（TPH）。秸秆的改性是通过在含有秸秆粉末的水溶液中，以亚甲基双丙烯酰胺作为交联剂，使苯乙烯和甲基丙烯酸丁酯原位共聚来实现的。与对照组（负载在未处理秸秆上的ODB）相比，实验组的结果表明，聚合物改性秸秆有利于微生物的生长。结果，TPH的降解率达到90.12%，分别比空白组（仅ODB）和对照组高50.54%和7.08%。对PMS - ODB转化特性的研究表明，低碳数、中碳数和高碳数烷烃的降解率均高于90%。测定实验组中TPH的（∑C）/（∑C）（高碳正构烷烃与低碳正构烷烃的质量比）、（pr）/（ph）（姥鲛烷/植烷的比值）和OEP（奇数碳正构烷烃与偶数碳正构烷烃的质量比）分别为0.6186、0.7248和1.4356，均为空白组、对照组和实验组中的最大值。这些发现表明，与对照组相比，秸秆改性可以增强对TPH的综合生物降解性能，即使是那些高度稳定的有机物，如碳链烷烃和类异戊二烯烃，这可能为以强化生物方式降解油类或有毒有机物开辟新的可能性。
