Zhang Danfeng, Chen Chen, Zhao Yan, Gao Yugang, Cai Enbo, Zhu Hongyan
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.
Nat Prod Res. 2021 Aug;35(16):2633-2639. doi: 10.1080/14786419.2019.1656622. Epub 2019 Aug 28.
A new 3,4--lupane triterpene, named sessiligenin (), along with four known 3,4--lupane triterpene derivatives (chiisanogenin , chiisanoside , divaroside , and sessiliside-A1 ) were isolated from the ethanol extract of the leaves of (Rupr. & Maxim.) S.Y. Hu by silica gel column chromatography, and their structures were determined by spectroscopic data. Furthermore, all these compounds were tested for their cytotoxicities against cancer cell lines HepG2, B16-F10, Lewis and YAC-1, as well as normal cell lines NCTC1469 and HL-7702, and significant cytotoxicities had been found for this new compound (sessiligenin ) which exhibited much lower cytotoxicities against normal cell lines NCTC1469 and HL-7702. It was deduced that the reduce of glycosyl from the structures of these 3,4--lupane triterpenoids enhanced the cytotoxicities. Furthermore, with the complete removal of glycosyl group and the 11-hydroxyl and 3-carboxyl formed by the opening of the lactone ring, the cytotoxicity increased significantly.
从（Rupr. & Maxim.）S.Y. Hu的叶子乙醇提取物中，通过硅胶柱色谱法分离得到一种新的3,4-羽扇豆烷三萜，命名为无柄皂苷元（），以及四种已知的3,4-羽扇豆烷三萜衍生物（千石皂苷元、千石皂苷、双瓦罗苷和无柄皂苷A1），并通过光谱数据确定了它们的结构。此外，对所有这些化合物针对癌细胞系HepG2、B16-F10、Lewis和YAC-1以及正常细胞系NCTC1469和HL-7702进行了细胞毒性测试，发现这种新化合物（无柄皂苷元）具有显著的细胞毒性，而其对正常细胞系NCTC1469和HL-7702的细胞毒性要低得多。据推断，这些3,4-羽扇豆烷三萜类化合物结构中糖基的减少增强了细胞毒性。此外，随着糖基的完全去除以及内酯环开环形成的11-羟基和3-羧基，细胞毒性显著增加。
