细辛乙醇提取物通过差异调控 p53 选择性诱导 G361 人黑色素瘤细胞凋亡。

Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53.

机构信息

Department of Anatomy and Cell Biology, School of Dentistry, Yangsan Campus of Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan-si, Gyeongsangnam, 50612, South Korea.

Department of Internal Medicine, School of Korean Medicine, Yangsan Campus of Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan-si, Gyeongsangnam, 50612, South Korea.

出版信息

BMC Complement Altern Med. 2019 Aug 28;19(1):231. doi: 10.1186/s12906-019-2609-2.

DOI:10.1186/s12906-019-2609-2

PMID:31462222

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6712601/

Abstract

BACKGROUND

In Korea and China, asiasari radix (AR) is widely used as a traditional anti-inflammatory and analgesic agent. After its skin-regenerating and hair loss-preventing activities were identified, several types of AR extracts were used for aesthetic purposes. Nevertheless, the effect of ARE on various types of skin cancers was not fully studied yet.

METHODS

In this study, we tested the effect of an ethanolic AR extract (ARE) on G361 human melanoma and HaCaT human keratinocyte cell lines. After ARE exposure, cell growth and the expression patterns of proteins and genes were monitored.

RESULTS

The ARE-mediated cell growth inhibition was greater in G361 cells than in HaCaT cells due to differences in its cell growth regulation effects. Interestingly, ARE treatment induced caspase-3-mediated apoptosis in G361 cells, but not in HaCaT cells. Furthermore, ARE reduced the expression of p53 and p21 proteins in G361 cells, whereas it induced their expression in HaCaT cells. ARE induced cell death in G361 cells through the reactive oxygen species (ROS)-dependent regulation of p53 and p21 in G361 cells. Microarray analysis showed that ARE regulates Mouse double minute 2 homolog (MDM2) and CASP8 and FADD-like apoptosis regulator (CFLAR) gene expression in G361 and HaCaT cells differently.

CONCLUSION

The treatment of ARE preferentially induces apoptosis in melanoma cells by the ROS-dependent differential regulation of p53 level. Therefore, ARE can be used as a new medicinal option for melanoma.

摘要

背景

在韩国和中国，白屈菜（AR）被广泛用作传统的抗炎和止痛剂。在确定其具有皮肤再生和防止脱发的作用后，几种类型的 AR 提取物被用于美容目的。然而，AR 对各种类型的皮肤癌的影响尚未得到充分研究。

方法

在这项研究中，我们测试了一种乙醇 AR 提取物（ARE）对 G361 人黑色素瘤和 HaCaT 人角质形成细胞系的作用。暴露于 ARE 后，监测细胞生长和蛋白质及基因的表达模式。

结果

由于其细胞生长调节作用的差异，ARE 介导的细胞生长抑制在 G361 细胞中比在 HaCaT 细胞中更大。有趣的是，ARE 处理诱导 G361 细胞中 caspase-3 介导的细胞凋亡，但不诱导 HaCaT 细胞凋亡。此外，ARE 降低了 G361 细胞中 p53 和 p21 蛋白的表达，而诱导了 HaCaT 细胞中它们的表达。ARE 通过 G361 细胞中 p53 和 p21 的活性氧（ROS）依赖性调节诱导 G361 细胞中的细胞死亡。微阵列分析表明，ARE 以不同的方式调节 G361 和 HaCaT 细胞中 Mouse double minute 2 homolog（MDM2）和 CASP8 和 FADD-like apoptosis regulator（CFLAR）基因的表达。