Yasumoto Y, Suga T, Miura M, Nomoto H, Tomino Y, Nomoto Y, Sakai H
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa-ken, Japan.
Ann Acad Med Singap. 1988 Jul;17(3):427-30.
Polyclonality of IgA production in IgA nephropathy (IgAN) was studied to elucidate the immunological aberrations in patients with this disease. The amounts of IgG, IgA and IgM and their kappa/lambda ratios in sera and supernatants of lymphocyte cultures were determined by the enzyme-linked immunosorbent assay. This method was sensitive enough to detect as little as 10% of monoclonal IgA in total IgA. The amount of IgA in IgAN was significantly increased in sera and supernatants without pokeweed-mitogen (PWM), but not changed in supernatants with PWM. There were no significant changes in the production of other immunoglobulins between IgAN and controls both in vivo and in vitro with and without addition of PWM. As far as the kappa/lambda ratios of immunoglobulins were concerned, no significant deviations were observed between IgAN and controls in any samples. These results indicated that increased IgA in patients with IgAN was polyclonal, supporting the concept of the heterogeneity of this disorder.
研究IgA肾病（IgAN）中IgA产生的多克隆性，以阐明该疾病患者的免疫异常。通过酶联免疫吸附测定法测定血清和淋巴细胞培养上清液中IgG、IgA和IgM的量及其κ/λ比值。该方法灵敏度足以检测到总IgA中低至10%的单克隆IgA。在无商陆有丝分裂原（PWM）的血清和上清液中，IgAN患者的IgA量显著增加，但在有PWM的上清液中无变化。在添加和不添加PWM的情况下，IgAN患者与对照组在体内和体外其他免疫球蛋白的产生均无显著变化。就免疫球蛋白的κ/λ比值而言，在任何样本中IgAN患者与对照组之间均未观察到显著偏差。这些结果表明，IgAN患者IgA增加是多克隆性的，支持了该疾病异质性的概念。
