Mann G E, Munoz M, Peran S
Department of Physiology, King's College London, U.K.
Alcohol. 1988 Sep-Oct;5(5):359-65. doi: 10.1016/0741-8329(88)90021-3.
The effects of ethanol on exocrine pancreatic amino acid transport and secretion were investigated during perfusion of the isolated rat pancreas with ethanol concentrations ranging from 0.06% to 4.1%. Amino acid transport was quantitated using a rapid dual isotope dilution technique in which unidirectional substrate uptake (15-20 sec) is assessed relative to an extracellular tracer. Pancreatic secretion evoked by 0.3 microM carbachol was abolished during perfusion with 0.32% ethanol. Influx of L-lysine, L-serine and methylaminoisobutyric acid (MeAIB) was marginally increased by 0.32% ethanol but significantly inhibited during subsequent perfusion with 1.28-4.1% ethanol. Pancreatic oxygen consumption and effluent PCO2 levels decreased with increasing ethanol concentration, and the control venous pH (7.21 +/- 0.01, n = 8) gradually approached arterial pH values (7.46 +/- 0.02, n = 9). These results indicate that low concentrations of ethanol readily inhibit secretagogue-induced pancreatic secretion. Amino acid transport at the basolateral membrane of the exocrine pancreatic epithelium appears only to be inhibited after acute exposure to high ethanol concentrations.
在用浓度范围为0.06%至4.1%的乙醇灌注离体大鼠胰腺的过程中，研究了乙醇对外分泌性胰腺氨基酸转运和分泌的影响。使用快速双同位素稀释技术对氨基酸转运进行定量，该技术通过相对于细胞外示踪剂评估单向底物摄取（15 - 20秒）来实现。在用0.32%乙醇灌注期间，由0.3微摩尔卡巴胆碱诱发的胰腺分泌被消除。0.32%乙醇使L - 赖氨酸、L - 丝氨酸和甲基氨基异丁酸（MeAIB）的流入量略有增加，但在随后用1.28 - 4.1%乙醇灌注期间显著受到抑制。胰腺耗氧量和流出液PCO2水平随乙醇浓度增加而降低，对照静脉pH值（7.21±0.01，n = 8）逐渐接近动脉pH值（7.46±0.02，n = 9）。这些结果表明，低浓度乙醇容易抑制促分泌剂诱导的胰腺分泌。外分泌性胰腺上皮细胞基底外侧膜的氨基酸转运似乎仅在急性暴露于高乙醇浓度后才受到抑制。
