Mann G E, Norman P S
Biochim Biophys Acta. 1984 Dec 19;778(3):618-22. doi: 10.1016/0005-2736(84)90415-2.
Relatively little is known about the hormonal regulation of amino acid transport in the normal and diabetic exocrine pancreas. In this study unidirectional influx and tracer efflux of L-serine at the basolateral interface of the rat pancreatic epithelium was investigated in the perfused exocrine pancreas using a rapid (less than 30 s) paired-tracer dilution technique. In the non-diabetic pancreas L-serine influx was saturable and stimulated by perfusion with exogenous bovine insulin (100 microU/ml). Transport of L-serine and methylaminoisobutyric acid was markedly elevated in pancreata isolated from streptozotocin diabetic rats and insulin partially reversed the stimulation of L-serine transport induced by experimental diabetes. These results suggest that insulin and diabetes modulate the epithelial transport activity for small neutral amino acids in the intact exocrine pancreas.
关于正常和糖尿病外分泌胰腺中氨基酸转运的激素调节，人们了解得相对较少。在本研究中，使用快速（少于30秒）的双标记示踪剂稀释技术，在灌注的外分泌胰腺中研究了大鼠胰腺上皮基底外侧界面处L-丝氨酸的单向流入和示踪剂流出。在非糖尿病胰腺中，L-丝氨酸流入是可饱和的，并受到外源性牛胰岛素（100微单位/毫升）灌注的刺激。从链脲佐菌素糖尿病大鼠分离的胰腺中，L-丝氨酸和甲基氨基异丁酸的转运明显升高，胰岛素部分逆转了实验性糖尿病诱导的L-丝氨酸转运刺激。这些结果表明，胰岛素和糖尿病调节完整外分泌胰腺中小中性氨基酸的上皮转运活性。
