College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Cell Tissue Res. 2020 Feb;379(2):291-300. doi: 10.1007/s00441-019-03090-y. Epub 2019 Aug 28.
Thyroid hormones (THs) are vital for normal reproductive function and dysregulation of TH impairs follicular development. Although the functions of THs on female reproduction are of great interest, the mechanisms still remain unclear. Many studies have shown that NO plays important roles in female reproduction. In the present study, we investigate the effects of TH dysregulation on nitric oxide synthase types (NOS) expression in rats. Propylthiouracil (PTU) and L-thyroxine were administered to rats to induce hypo- and hyperthyroidism, respectively. Ovarian histology was detected by immunohistochemistry (IHC) and protein or mRNA content was analyzed by Western blotting or RT-PCR, respectively. The results showed that NOS1, NOS2 and NOS3 expressions were detected in the oocyte, granulosa cell and theca cell in all follicular stages, which were up-regulated by eCG treatment. NOS1 protein content was increased in both PTU and L-thyroxine treatments. There were no significant differences in NOS2 levels between the treatment and the control group. However, NOS3 was only increased in the hyperthyroid group. These results were consistent with the IHC staining. The present study provides evidence that TH dysregulation alters NOSs profiles, which suggests that NOSs/nitric oxide (NO) is possibly involved in the regulation of female reproduction.
甲状腺激素（THs）对正常生殖功能至关重要，TH 的失调会损害卵泡发育。尽管 TH 对女性生殖的功能非常重要，但机制仍不清楚。许多研究表明，NO 在女性生殖中发挥着重要作用。在本研究中，我们研究了 TH 失调对大鼠中一氧化氮合酶类型（NOS）表达的影响。给予丙硫氧嘧啶（PTU）和左甲状腺素分别诱导甲状腺功能减退和甲状腺功能亢进。通过免疫组织化学（IHC）检测卵巢组织学，通过 Western blot 或 RT-PCR 分别分析蛋白质或 mRNA 含量。结果表明，NOS1、NOS2 和 NOS3 在所有卵泡阶段的卵母细胞、颗粒细胞和膜细胞中均有表达，经 eCG 处理后表达上调。PTU 和左甲状腺素处理后 NOS1 蛋白含量增加。NOS2 水平在治疗组与对照组之间无显著差异。然而，NOS3 仅在甲状腺功能亢进组中增加。这些结果与 IHC 染色一致。本研究提供了证据表明，TH 失调改变了 NOSs 谱，提示 NOSs/一氧化氮（NO）可能参与了女性生殖的调节。
