Bakırhan Elfide Gizem, Kaplan Süleyman
Department of Histology and Embryology, Faculty of Medicine, Adıyaman University, Adıyaman, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey.
Histochem Cell Biol. 2025 Jul 3;163(1):72. doi: 10.1007/s00418-025-02399-w.
This study investigated the effects of an experimentally induced hyperthyroidism model on the pituitary-ovarian axis and the possible protective role of vitamin E against these adverse effects. Wistar albino rats were divided into five groups of eight animals each: control ("Cont," no any treatment); hyperthyroidism ("Hypert," L-thyroxine at 0.3 mg/kg/day); "Sham" (1 ml/day corn oil); "Hypert + vit E" (L-thyroxine (0.3 mg/kg/day and 100 mg/kg/day/1 ml vitamin E); and "Vit E" (100 mg/kg/day/1 ml vitamin E) groups. At the end of the experiment, ovarian tissues were exposed to electron microscopic and stereological analyses. Thyroid stimulating hormone (TSH), free thyroxine (FT4), follicle stimulating hormone (FSH), luteinizing hormone (LH), and superoxide dismutase (SOD) levels, as well as catalase (CAT) enzyme activity, were also determined in blood serum samples. Ovarian follicle numbers and volumes; corpus luteum and cortex volume; body weights; and TSH, FSH, and SOD levels decreased significantly in the Hypert group compared with the Cont group. However, connective tissue volume, CAT enzyme activity, and FT4 levels increased in the Hypert group compared with the Cont group. Vitamin E supplementation was observed to play a protective role on antral follicle, corpus luteum, and connective tissue volumes, CAT activity, and SOD and FSH levels. Hyperthyroidism reduces the number of ovarian follicles and may cause infertility problems. The adverse effect of hyperthyroidism on the pituitary-ovarian axis can be ameliorated by means of vitamin E, since this may have a homeostatic effect on this axis, as shown by the biochemical, histopathological, and stereological analyses in this study.
本研究调查了实验性诱导的甲状腺功能亢进模型对垂体 - 卵巢轴的影响以及维生素E对这些不良反应可能的保护作用。将Wistar白化大鼠分为五组，每组八只动物：对照组（“Cont”，未进行任何处理）；甲状腺功能亢进组（“Hypert”，每天0.3mg/kg的L - 甲状腺素）；“假手术组”（每天1ml玉米油）；“Hypert + 维生素E组”（L - 甲状腺素（0.3mg/kg/天）和100mg/kg/天/1ml维生素E）；以及“维生素E组”（100mg/kg/天/1ml维生素E）。实验结束时，对卵巢组织进行电子显微镜和体视学分析。还测定了血清样本中的促甲状腺激素（TSH）、游离甲状腺素（FT4）、促卵泡激素（FSH）、黄体生成素（LH）和超氧化物歧化酶（SOD）水平以及过氧化氢酶（CAT）活性。与对照组相比，甲状腺功能亢进组的卵巢卵泡数量和体积、黄体和皮质体积、体重以及TSH、FSH和SOD水平显著降低。然而，与对照组相比，甲状腺功能亢进组的结缔组织体积、CAT酶活性和FT4水平升高。观察到补充维生素E对窦卵泡、黄体和结缔组织体积、CAT活性以及SOD和FSH水平具有保护作用。甲状腺功能亢进会减少卵巢卵泡数量并可能导致不孕问题。本研究中的生化、组织病理学和体视学分析表明，维生素E可改善甲状腺功能亢进对垂体 - 卵巢轴的不良影响，因为它可能对该轴具有稳态作用。
