Visch Wouter, Rad-Menéndez Cecilia, Nylund Göran M, Pavia Henrik, Ryan Matthew J, Day John
Department of Marine Sciences, Tjärnö Marine Laboratory, University of Gothenburg, Strömstad, Sweden.
Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Oban, United Kingdom.
Biopreserv Biobank. 2019 Oct;17(5):378-386. doi: 10.1089/bio.2018.0147. Epub 2019 Aug 29.
Sugar kelp () is an economically important species, and natural populations provide diverse and productive habitats as well as important ecosystem services. For seaweed aquaculture to be successful in newly emerging industry in Europe and other Western countries, it will have to develop sustainable production management strategies. A key feature in this process is the capacity to conserve genetic diversity for breeding programs aimed at developing seed stock for onward cultivation, as well as in the management of wild populations, as potentially interesting genetic resources are predicted to disappear due to climate change. In this study, the cryopreservation of male and female gametophytes (haploid life stage) of by different combinations of two-step cooling methods and cryoprotectants was explored. We report here that cryopreservation constitutes an attractive option for the long-term preservation of gametophytes, with viable cells in all treatment combinations. The highest viabilities for both male and female gametophytes were found using controlled-rate cooling methods combined with dimethyl sulfoxide 10% (v/v). Morphological normal sporophytes were observed to develop from cryopreserved vegetative gametophytic cells, independent of treatment. This indicates that cryopreservation is a useful preservation method for male and female gametophytes.
糖海带（）是一种具有重要经济价值的物种，其天然种群提供了多样且富有生产力的栖息地以及重要的生态系统服务。对于海藻养殖在欧洲和其他西方国家成为新兴产业而言，必须制定可持续的生产管理策略。这一过程的一个关键特征是有能力为育种计划保存遗传多样性，这些育种计划旨在培育用于后续养殖的种苗，同时也用于野生种群的管理，因为预计由于气候变化，潜在有趣的遗传资源将会消失。在本研究中，探索了通过两步冷却方法和冷冻保护剂的不同组合对糖海带雄配子体和雌配子体（单倍体生活阶段）进行冷冻保存。我们在此报告，冷冻保存是长期保存糖海带配子体的一个有吸引力的选择，所有处理组合中均有活细胞。使用程序降温法结合10%（v/v）二甲基亚砜时，雄配子体和雌配子体的存活率最高。观察到从冷冻保存的营养性配子体细胞发育出形态正常的孢子体，且与处理无关。这表明冷冻保存是糖海带雄配子体和雌配子体的一种有用的保存方法。
