Department of Biochemistry, Faculty of Science and Arts, Igdır University, 76100, Igdır, Turkey.
Department of Nursing, School of Health, Muş Alparslan University, 49250, Mus, Turkey.
Chem Biodivers. 2019 Oct;16(10):e1900341. doi: 10.1002/cbdv.201900341. Epub 2019 Sep 18.
The aim of this work was to investigate the enzyme inhibition, antioxidant activity, and phenolic compounds of Lecokia cretica (Lam.) DC. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase enzymes were strongly inhibited by the L. cretica extracts. IC values for the three enzymes were found as 3.21 mg/mL, 2.1 mg/mL, and 2.07 mg/mL, respectively. Antioxidant activities were examined in both aqueous and ethanol (EtOH) extracts using CUPRAC, FRAP, and DPPH method. Also, the phenolic compounds of the endemic plant were identified and quantified by using HPLC/MS/MS. According to the results, the extracts have remarkable antioxidant activities. The most abundant phenolic acids of L. cretica in EtOH extract were determined as quinic acid (12.76 mg/kg of crude extract), chlorogenic acid (3.39 mg/kg), and malic acid (2.38 mg/kg).
本工作旨在研究 Lecokia cretica（Lam.）DC 的酶抑制、抗氧化活性和酚类化合物。乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和α-糖苷酶被 L. cretica 提取物强烈抑制。三种酶的 IC 值分别为 3.21mg/mL、2.1mg/mL 和 2.07mg/mL。使用 CUPRAC、FRAP 和 DPPH 法分别在水提物和乙醇（EtOH）提取物中测定抗氧化活性。此外，还使用 HPLC/MS/MS 对该特有植物的酚类化合物进行了鉴定和定量。结果表明，提取物具有显著的抗氧化活性。EtOH 提取物中 L. cretica 的主要酚酸分别为奎尼酸（12.76mg/kg 粗提物）、绿原酸（3.39mg/kg）和苹果酸（2.38mg/kg）。
