Gastrointestinal Immunology Group, Department of Immunobiology, Institute of Biology, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil; Graduation Program in Pathology, School of Medicine, Antônio Pedro University Hospital, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil.
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.
Exp Mol Pathol. 2019 Dec;111:104302. doi: 10.1016/j.yexmp.2019.104302. Epub 2019 Aug 26.
Assessing the gut mucosa milieu is important to grade the inflammatory process in conditions such as food hypersensitivity, allergy, gut parasitosis, etc. However, the gastrointestinal tract comprises a challenging system to evaluate, due to its thin tubular structure and mucosa, which suffer fast autolysis after death. Irrespective of the preferred inflammatory score system, it is important to choose the technique that will render the best tissue analysis. Thus, our aim was to compare two of the most frequently used methods to collect, process and analyze gut segments, the Swiss Roll and the Intestinal Strips. Normal C57Bl/6 mice were randomly assigned to Rolls or Strips group. After an overdose of anesthetics, segments of the duodenum, jejunum and ileum were collected and prepared accordingly for histological processing and analysis. Our results show the villi in the Rolls tend to be shorter and wider than those in the Strips in the duodenum and jejunum but not the ileum. No significant differences were observed in intra-epithelial lymphocytes and goblet cells counts. Finally, we staged each segment using our histomorphometric classification system, which revealed that although all animals presented a normal intestinal mucosa, those assigned to the Rolls group had their mucosa staged in the Infiltrative Stage while the Strips group had their mucosa staged as Normal. In conclusion, Swiss Rolls might be desirable for a wider assessment of the intestine, as it allows large segments to be analyzed at once, while Strips are better suited when detailed evaluation of each villus is intended.
评估肠道黏膜环境对于评估食物过敏、过敏、肠道寄生虫病等情况下的炎症过程非常重要。然而，由于胃肠道的管状结构和黏膜很薄，死后会迅速自溶，因此评估胃肠道是一个具有挑战性的系统。无论首选的炎症评分系统如何，选择能够提供最佳组织分析的技术都很重要。因此，我们的目的是比较两种最常用于收集、处理和分析肠道段的方法，即瑞士卷和肠条。正常 C57Bl/6 小鼠被随机分配到 Rolls 或 Strips 组。在过量麻醉后，收集十二指肠、空肠和回肠的肠段，并相应地进行组织学处理和分析。我们的结果表明，在十二指肠和空肠中，Rolls 中的绒毛比 Strips 中的更短、更宽，但在回肠中并非如此。上皮内淋巴细胞和杯状细胞计数无显著差异。最后，我们使用我们的组织形态计量分类系统对每个肠段进行分期，结果表明，尽管所有动物的肠道黏膜均正常，但分配到 Rolls 组的动物的黏膜处于浸润期，而 Strips 组的黏膜处于正常期。总之，瑞士卷可能更适合对肠道进行更广泛的评估，因为它可以一次分析较大的肠段，而当需要对每个绒毛进行详细评估时，肠条则更为合适。
