CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266000, China.
Fish Shellfish Immunol. 2019 Nov;94:17-26. doi: 10.1016/j.fsi.2019.08.057. Epub 2019 Aug 26.
Meretrix petechialis is an important commercial aquaculture species in China. During the clam culture period, mass mortality events often occurred due to the Vibrio infection. In this paper, M. petechialis were challenged with Vibrio parahaemolyticus immersion to simulate a natural infection, and the infection process were divided into four phases including latency, prodrome, onset and recovery phases based on the clam mortality data. Then, the dynamic response of clams to Vibrio infection at different infection phases were investigated by transcriptome analysis. A total of 38,067 differentially expressed genes (DEGs) were identified at different infection phases. DEG annotations showed that immune-related and metabolism-related signaling pathways were enriched, indicating that immune defense and metabolism process play key roles during bacterial infection. Three kinds of expression pattern were classified by cluster analysis, including U-shape, L-shape and inverted V-shape. Anabolism and cellular growth proliferation related signaling pathways were repressed (long-lasting or transient) during bacterial infection. However, the immune related signaling pathways with different immune functions showed induction expression or repression expression against bacterial infection, which indicated that immune system take different strategies against bacterial infection. Furthermore, some signaling pathways such as PI3K-Akt signaling pathway both involved in immune defense and cell metabolism. This study provides a sight that the dynamic immunity and metabolic responses may be integrated to improve the host survival and shift more energy for immune defense.
斑节对虾是中国重要的海水养殖品种。在养殖过程中，常因弧菌感染而导致大规模死亡。本研究通过斑节对虾弧菌浸泡感染的方式来模拟自然感染，并根据死亡率数据将感染过程分为潜伏期、前驱期、发病期和恢复期四个阶段。然后，通过转录组分析研究了对虾在不同感染阶段对弧菌感染的动态反应。在不同感染阶段共鉴定到 38067 个差异表达基因（DEGs）。DEG 注释表明，免疫相关和代谢相关信号通路富集，表明免疫防御和代谢过程在细菌感染过程中发挥关键作用。通过聚类分析将三种表达模式分为 U 型、L 型和倒 V 型。在细菌感染过程中，与合成代谢和细胞生长增殖相关的信号通路受到抑制（持续或短暂）。然而，具有不同免疫功能的免疫相关信号通路对细菌感染表现出诱导表达或抑制表达，这表明免疫系统针对细菌感染采取了不同的策略。此外，一些信号通路，如 PI3K-Akt 信号通路，既参与免疫防御，也参与细胞代谢。本研究提供了一个视角，即动态免疫和代谢反应可能被整合以提高宿主的存活率，并转移更多的能量用于免疫防御。
