Song Ha Eun, Lee Hyo Yeong, Kim Su Jung, Back Sung Hoon, Yoo Hyun Ju
Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Metabolites. 2019 Aug 28;9(9):173. doi: 10.3390/metabo9090173.
Short chain fatty acids (SCFAs) are the main products of dietary fibers that are not digested by the human body, and they have been shown to affect human metabolism and inflammation. The amount of SCFAs in the body is related to many human diseases, and studies have focused on elucidating their roles and target molecules in both metabolic and immune responses. Thus, the quantitation of SCFAs in biological samples becomes crucial in understanding their important roles in the human body. Herein, a facile profiling method of SCFAs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and then applied to biological samples. C2-C6 SCFAs were derivatized while using 4-acetamido-7-mercapto-2,1,3-benzoxadiazole for 5 min. at room temperature prior to LC-MS/MS analysis, and characteristic fragmentation patterns and increased hydrophobicity after chemical derivatization enabled specific discrimination among 12 SCFAs. Derivatization was fast and reliable, and the reaction products were stable for a week at 4 °C. The developed method was applied to measure SCFAs in mouse feces, plasma, and human exhaled breath condensates. This fast and simple method can save labor and effort to profile SCFAs from various biological samples.
短链脂肪酸（SCFAs）是膳食纤维在人体中未被消化的主要产物，并且已被证明会影响人体代谢和炎症反应。体内短链脂肪酸的含量与许多人类疾病相关，研究主要集中在阐明它们在代谢和免疫反应中的作用及靶分子。因此，对生物样品中短链脂肪酸进行定量分析对于理解它们在人体中的重要作用至关重要。在此，开发了一种使用液相色谱-串联质谱法（LC-MS/MS）对短链脂肪酸进行简便分析的方法，并将其应用于生物样品。在进行LC-MS/MS分析之前，使用4-乙酰氨基-7-巯基-2,1,3-苯并恶二唑在室温下将C2-C6短链脂肪酸衍生化5分钟，化学衍生化后的特征性裂解模式和增加的疏水性使得能够对12种短链脂肪酸进行特异性区分。衍生化快速且可靠，反应产物在4℃下可稳定保存一周。所开发的方法用于测定小鼠粪便、血浆和人类呼出气冷凝物中的短链脂肪酸。这种快速简便的方法可以节省从各种生物样品中分析短链脂肪酸的人力和精力。
