Department of Hydrobiology and Protection of Ecosystems, University of Life Sciences in Lublin, Dobrzańskiego 37, 20-262, Lublin, Poland.
Department of Hydrobiology and Protection of Ecosystems, University of Life Sciences in Lublin, Dobrzańskiego 37, 20-262, Lublin, Poland.
J Therm Biol. 2019 Aug;84:154-163. doi: 10.1016/j.jtherbio.2019.06.012. Epub 2019 Jul 2.
L-proline (L-PROL) is an essential amino acid, a constituent of many proteins and the osmoprotective molecule produced and accumulated in higher plants and some freshwater microalgae in response to various environmental stressors. Knowledge on thermoprotective effects of this amino acid on freshwater invertebrates is very scarce. Therefore the aim of our study was to determine the effect of L-PROL at concentrations: 10 mg/L, 20 mg/L and 50 mg/L on swimming behavior (immobilization, swimming track density, swimming speed, turning ability) of Daphnia magna subjected to temperatures: 22 °C, 35 °C and 38 °C. We found that L-PROL elevated all the measured swimming parameters at 22 °C when compared to the untreated crustaceans. Furthermore, L-PROL alleviated heat-induced inhibition of these parameters in the experimental animals subjected to 35 °C. The results suggest that L-PROL stimulates swimming performance and alleviates alterations of swimming parameters induced by heat stress in D. magna. Moreover, these findings may support the hypothesis that in natural conditions, L-PROL may protect crustaceans against thermal stress.
L-脯氨酸（L-PROL）是一种必需氨基酸，是许多蛋白质的组成部分，也是高等植物和一些淡水微藻在应对各种环境胁迫时产生和积累的渗透保护分子。关于这种氨基酸对淡水无脊椎动物的热保护作用的知识非常有限。因此，我们的研究目的是确定 L-PROL 在以下浓度下对大型溞游泳行为（固定、游泳轨迹密度、游泳速度、转弯能力）的影响：10mg/L、20mg/L 和 50mg/L，温度分别为：22°C、35°C 和 38°C。我们发现，与未处理的甲壳类动物相比，L-PROL 在 22°C 时提高了所有测量的游泳参数。此外，L-PROL 缓解了实验动物在 35°C 下受到的热诱导对这些参数的抑制。研究结果表明，L-PROL 刺激了大型溞的游泳性能，并缓解了热应激引起的游泳参数变化。此外，这些发现可能支持这样一种假设，即在自然条件下，L-PROL 可能保护甲壳类动物免受热应激。
