Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, Gyeongnam.
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul.
Diagn Microbiol Infect Dis. 2019 Dec;95(4):114872. doi: 10.1016/j.diagmicrobio.2019.114872. Epub 2019 Jul 26.
Detection of carbapenemase-producing Enterobacteriaceae (CPE) has become critical for appropriate antimicrobial therapy and for controlling the spread of infection. We evaluated Triton Hodge test (THT) for screening CPE. A spreader can be used to apply more constant volume of Triton on whole surface of Mueller-Hinton agar (MHA), or alternatively, a 10-μL inoculating loop can be used to apply a 20% Triton solution lineally. The THT procedure can be simplified by eliminating the 1/10 dilution step of indicator bacteria from the McFarland 0.5 turbidity suspension. The presence of Triton in the MHA plates significantly increased the enhanced growth size of not only Enterobacteriaceae producing NDM-1-like enzymes but also those producing the most prevalent KPC-2-like enzyme, resulting in 100% sensitivity of the test.
检测产碳青霉烯酶肠杆菌科（CPE）对于选择适当的抗菌治疗和控制感染的传播变得至关重要。我们评估了 Triton Hodge 试验（THT）用于筛查 CPE。可以使用散布器在 Mueller-Hinton 琼脂（MHA）的整个表面施加更恒定体积的 Triton，或者可以使用 10μL 的接种环在线性施加 20%的 Triton 溶液。通过从 McFarland 0.5 浊度悬浮液的指示菌中消除 1/10 的稀释步骤，可以简化 THT 程序。MHA 平板中的 Triton 的存在显著增加了不仅产生 NDM-1 样酶而且产生最常见的 KPC-2 样酶的肠杆菌科的增强生长大小，导致该试验的敏感性达到 100%。
