Teethaisong Yothin, Hobbs Glyn, Nakouti Ismini, Evans Katie, Eumkeb Griangsak
School of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, United Kingdom.
Diagn Microbiol Infect Dis. 2018 May;91(1):85-88. doi: 10.1016/j.diagmicrobio.2017.12.023. Epub 2018 Jan 6.
Reliable, simple and rapid methods for laboratory detection of carbapenemases are important for an appropriate antibiotic administration. A nitrocefin disc containing ertapenem for rapid screening of carbapenemase production among Enterobacteriaceae is developed in the present study. A total of 87 molecularly-confirmed Enterobacteriaceae including 31 carbapenemase producers and 56 non-carbapenemase producers, were tested with nitrocefin discs supplemented with and without ertapenem (20 μg/disc). Nitrocefin discs with ertapenem successfully discriminated all 31 carbapenemase and all non-carbapenemase producers within 30 minutes. The sensitivity and specificity of the method were 100%. The minimum inhibitory concentrations (MICs) of ertapenem against all carbapenemase-producing isolates ranged from 1 to ≥256 μg/mL. This simple test could help to minimize the treatment failure and control the dissemination of infections caused by carbapenemase-associated resistant bacteria. It is a promising approach that could be performed routinely in any laboratory.
可靠、简单且快速的碳青霉烯酶实验室检测方法对于合理使用抗生素至关重要。本研究开发了一种含厄他培南的硝基头孢菌素纸片，用于快速筛查肠杆菌科细菌中的碳青霉烯酶产生情况。共对87株经分子鉴定的肠杆菌科细菌进行了检测，其中包括31株碳青霉烯酶产生菌和56株非碳青霉烯酶产生菌，分别用添加和未添加厄他培南（20μg/片）的硝基头孢菌素纸片进行检测。含厄他培南的硝基头孢菌素纸片在30分钟内成功鉴别出所有31株碳青霉烯酶产生菌和所有非碳青霉烯酶产生菌。该方法的敏感性和特异性均为100%。厄他培南对所有产碳青霉烯酶分离株的最低抑菌浓度（MIC）范围为1至≥256μg/mL。这种简单的检测方法有助于减少治疗失败，并控制由碳青霉烯酶相关耐药菌引起的感染传播。这是一种有前景的方法，可在任何实验室常规开展。
