Kato Ladies Clinic, 7-20-3 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan.
Sci Rep. 2019 Aug 29;9(1):12519. doi: 10.1038/s41598-019-48918-3.
In natural cycle or minimal stimulation cycle IVF, buserelin acetate (buserelin), a gonadotropin-releasing hormone agonist, is often used as a maturation trigger; however, its effect on pregnancy outcomes remains unclear. Therefore, in the present study, we compared uterine receptivity in buserelin-administered mice with that in human chorionic gonadotropin (hCG)-administered mice during the peri-implantation period. Implantation, decidualisation, and term-pregnancy were impaired following hCG, but not buserelin administration. hCG stimulated the synthesis and secretion of progesterone and oestradiol, whereas ovarian steroidogenesis in the buserelin-treated group was comparable with that in the control group. Furthermore, similar to the observation in controls, the buserelin-treated group exhibited activation of progesterone receptor signalling and inhibition of oestrogen receptor signalling in the endometrial epithelium on the day of implantation. However, epithelial progesterone signalling was not detected, and a high expression of genes downstream to oestrogen was observed on day 4 following hCG administration. These results suggest that buserelin administration does not impact uterine receptivity as it did not affect ovarian steroidogenesis and endometrial steroid signalling. Therefore, buserelin is preferred as an oocyte maturation trigger to optimise uterine receptivity during treatments involving timed intercourse, intrauterine insemination, or fresh embryo transfer following in vitro fertilisation.
在自然周期或微刺激周期的试管婴儿中，常使用促性腺激素释放激素激动剂醋酸布舍瑞林（buserelin）作为成熟触发剂；然而，其对妊娠结局的影响尚不清楚。因此，在本研究中，我们比较了在着床期使用人绒毛膜促性腺激素（hCG）和布舍瑞林对小鼠子宫容受性的影响。hCG 处理会损害着床、蜕膜化和足月妊娠，但布舍瑞林处理不会。hCG 刺激孕激素和雌二醇的合成和分泌，而布舍瑞林处理组的卵巢类固醇生成与对照组相当。此外，与对照组的观察结果相似，布舍瑞林处理组在着床日的子宫内膜上皮中表现出孕激素受体信号的激活和雌激素受体信号的抑制。然而，上皮孕激素信号未被检测到，并且在 hCG 处理后第 4 天观察到雌激素下游基因的高表达。这些结果表明，布舍瑞林处理不会影响子宫容受性，因为它不会影响卵巢类固醇生成和子宫内膜类固醇信号。因此，布舍瑞林作为卵母细胞成熟触发剂，在涉及定时性交、宫腔内人工授精或体外受精后新鲜胚胎移植的治疗中，可优化子宫容受性。
