Bao Xing-Qi, Huang Yi-Chen, Chen Fang
Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, 200062, China.
Shanghai 6th People's Hospital, Shanghai Jiao Tong University, Shanghai, 200233, China.
Evid Based Complement Alternat Med. 2019 Jul 29;2019:8424872. doi: 10.1155/2019/8424872. eCollection 2019.
To explore the effect of C-phycocyanin (C-PC) on voiding behavior and histological changes in cyclophosphamide- (CYP-) induced cystitis in mice.
Sixty female mice were included. The mice in the C-PC group received C-PC (25 mg/kg, twice, i.p.) and then CYP (200 mg/kg, i.p.) two hours later, while the mice in the CYP group only received the equivalent CYP. Saline was injected in the mice in the control group. A voided stain on paper (VSOP) test was conducted to analyze the micturition. The bladders were harvested for histological evaluation and measurements of inflammatory factors.
C-PC reduced the micturition frequency in the mice with CYP-induced cystitis. The bladder/body weight ratio and edema were remarkably higher in the CYP group compared to the C-PC group. C-PC suppressed the expressions of COX-2, PGE, and EP4 (prostaglandin E receptor 4) according to the ELISA assay. Immunohistochemical staining also indicated that C-PC reduced the expressions of COX-2 in urothelium and EP4 in smooth muscles.
C-PC relieved symptoms associated with CYP-induced cystitis in mice by inhibiting bladder inflammation through COX-2 and EP4 expression.
探讨藻蓝蛋白（C-PC）对环磷酰胺（CYP）诱导的小鼠膀胱炎排尿行为及组织学变化的影响。
纳入60只雌性小鼠。C-PC组小鼠先腹腔注射C-PC（25mg/kg，2次），2小时后腹腔注射CYP（200mg/kg），而CYP组小鼠仅注射等量的CYP。对照组小鼠注射生理盐水。进行纸上排尿印记（VSOP）试验以分析排尿情况。采集膀胱进行组织学评估及炎症因子测定。
C-PC降低了CYP诱导的膀胱炎小鼠的排尿频率。与C-PC组相比，CYP组的膀胱/体重比及水肿明显更高。酶联免疫吸附测定显示C-PC抑制了环氧化酶-2（COX-2）、前列腺素E（PGE）和前列腺素E受体4（EP4）的表达。免疫组织化学染色也表明C-PC降低了尿路上皮中COX-2及平滑肌中EP4的表达。
C-PC通过抑制COX-2和EP4表达减轻膀胱炎症，从而缓解CYP诱导的小鼠膀胱炎相关症状。
