Al Sayed Rahaf, Smith Whitnei, Rogers Nicole, Smith Nuri, Clark Daniel, Castillo Gabriel, McLeod Hunter, Glenister Stewart, Shorter Kimberly R
University of South Carolina Upstate Division of Natural Sciences and Engineering, 800 University Way, Spartanburg, SC, 29303, USA.
Biochem Biophys Rep. 2019 Aug 17;20:100681. doi: 10.1016/j.bbrep.2019.100681. eCollection 2019 Dec.
Many diseases are now associated with aberrant epigenetics and gene expression changes. Epigenetics can be modified by factors like diet. One dietary factor, folic acid, is consumed in various forms including supplements, energy drinks, and fortified grains. It was hypothesized high levels of folic acid would affect gene expression and enzyme activity of chromatin modifying enzymes as well as dendritic spine densities in a commonly utilized neuron model, the SHSY5Y cell. Decreased MBD2 and MECP2 were discovered upon treatment of SHSY5Y cells with a 2x folic acid dose. Corresponding decreases in dendritic spines were apparent in the 2x folic acid treated cells as well. Activity of DNMTs and H3K4 HMTs was altered. Further, H3K4me1, H3K4me3, H3K9Ac, and global DNA methylation were decreased in the 2x folic acid treated cells. Further studies are warranted to determine if the effects of excess folic acid are detrimental to organismal physiology.
现在许多疾病都与异常的表观遗传学和基因表达变化有关。表观遗传学可以被饮食等因素所修饰。一种饮食因素，即叶酸，以多种形式被摄入，包括补充剂、能量饮料和强化谷物。据推测，高剂量叶酸会影响常用神经元模型SHSY5Y细胞中染色质修饰酶的基因表达和酶活性以及树突棘密度。用两倍叶酸剂量处理SHSY5Y细胞后，发现MBD2和MECP2减少。在两倍叶酸处理的细胞中，树突棘也相应减少。DNMTs和H3K4 HMTs的活性发生了改变。此外，两倍叶酸处理的细胞中H3K4me1、H3K4me3、H3K9Ac和整体DNA甲基化水平降低。有必要进行进一步研究以确定过量叶酸的影响是否对机体生理有害。
