Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
Eat Weight Disord. 2020 Oct;25(5):1321-1332. doi: 10.1007/s40519-019-00765-x. Epub 2019 Aug 29.
Genetic variants determine the predisposition of an individual to obesity in a given environment. The present study was conducted to seek an association of the FTO variant rs1421085 with overweight/obesity and related traits in 612 Pakistani subjects in a case-control manner (overweight/obese = 306 and non-obese = 306). Moreover, interaction effects of the rs1421085 and overweight/obesity on multiple metabolic traits were also investigated, which were never explored before in Pakistani as well as in any other population.
Anthropometric traits were measured by standard procedures, while metabolic parameters were determined by biochemical assays. Genotyping of the rs1421085 was carried out by TaqMan allelic discrimination assay. The data were analysed using SPSS software version 19.
The study revealed a significant association of the rs1421085 with overweight/obese phenotype with respect to over-dominant model indicated by h-index. The CT genotype of the rs1421085 was observed to increase the risk of being overweight/obese by 1.583 times (95% CI 1.147-2.185, p = 0.005). The CT genotype was also found to be associated with higher values of all anthropometric variables (except height and waist-to-hip ratio). Moreover, the interaction between the CT genotype of the rs1421085 and overweight/obesity was found to influence several metabolic parameters (raised blood pressure, product of triglyceride and glucose index, triglyceride levels, LDL-C, VLDL-C, coronary risk index, atherogenic index, and triglyceride-to-HDL-C ratio).
In conclusion, the rs1421085 was found to be associated with overweight/obesity and related anthropometric traits independent of age and gender in Pakistani population. Moreover, this variant was found to influence various metabolic traits in the presence of overweight/obesity.
Level III, case-control analytic study.
遗传变异决定了个体在特定环境下肥胖的易感性。本研究旨在通过病例对照研究（超重/肥胖组=306 例，非肥胖组=306 例），探讨 FTO 变异 rs1421085 与巴基斯坦人群超重/肥胖及相关特征的相关性。此外，还研究了 rs1421085 与超重/肥胖之间的相互作用对多种代谢特征的影响，这在巴基斯坦人和其他人群中以前从未探索过。
采用标准程序测量人体测量学特征，采用生化分析方法测定代谢参数。采用 TaqMan 等位基因鉴别检测法对 rs1421085 进行基因分型。数据分析采用 SPSS 软件版本 19。
研究表明，rs1421085 与超重/肥胖表型存在显著关联，超显性模型的 h 指数表明 CT 基因型增加超重/肥胖的风险增加 1.583 倍（95%CI 1.147-2.185，p=0.005）。还发现 rs1421085 的 CT 基因型与所有人体测量变量（身高和腰臀比除外）的较高值相关。此外，还发现 rs1421085 的 CT 基因型与超重/肥胖之间的相互作用会影响多种代谢参数（血压升高、甘油三酯和葡萄糖指数乘积、甘油三酯水平、LDL-C、VLDL-C、冠心病风险指数、致动脉粥样硬化指数和甘油三酯/高密度脂蛋白胆固醇比值）。
总之，在巴基斯坦人群中，rs1421085 与超重/肥胖及相关的人体测量特征有关，与年龄和性别无关。此外，在超重/肥胖的情况下，该变体被发现会影响各种代谢特征。
三级，病例对照分析研究。
