Shanghai Innovation Center of Traditional Chinese Medicine Health Service, School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Shanghai Jiao Tong University, Shanghai, China.
BMC Endocr Disord. 2023 Jan 10;23(1):11. doi: 10.1186/s12902-022-01234-w.
We investigated the prevalence and clinical metabolic characteristics of lean nonalcoholic fatty liver disease (NAFLD) in an elderly Chinese population and assessed the relevance of lipid markers and genetic variation. All 5,338 community subjects underwent detailed clinical and laboratory examinations and were divided into three groups: lean (Body mass index (BMI) < 23 kg/m, n = 2,012), overweight (BMI = 23-24.9 kg/m, n = 1,354), and obese (BMI ≥ 25 kg/m, n = 1,972). Single nucleotide polymorphisms were selected based on those reported in previous NAFLD or obesity genome-wide association studies. The frequencies of alleles and genotypes were calculated and statistically analyzed with Pearson's χ tests. One-way ANCOVA was used to test the association between positive SNPs and metabolic parameters in lean NAFLD individuals. Our results showed that the C allele frequency of rs2279026, the G allele of rs2279028, the C allele of rs780093, and the C allele frequency of rs1260326 were higher in obese NAFLD than in lean NAFLD (P < 0.05). In addition, we observed an association between the CC of rs1421085, TT of rs3751812, AA of rs8050136, and AA of rs9939609 genotypes in the FTO gene and low-density lipoprotein levels (P < 0.05). In conclusion, our findings provide a unique perspective on the prevalence, genetic characteristics, and metabolic profile of NAFLD in older lean individuals in China. This is the first study to examine the association between genetic variants in the FTO, TFAP2B and GCKR genes and NAFLD in a cohort of lean individuals.
我们调查了老年中国人群中瘦型非酒精性脂肪性肝病（NAFLD）的流行率和临床代谢特征，并评估了脂质标志物和遗传变异的相关性。所有 5338 名社区受试者均接受了详细的临床和实验室检查，并分为三组：瘦型（BMI<23kg/m2，n=2012）、超重（BMI=23-24.9kg/m2，n=1354）和肥胖（BMI≥25kg/m2，n=1972）。根据先前的 NAFLD 或肥胖全基因组关联研究中报道的单核苷酸多态性选择单核苷酸多态性。计算等位基因和基因型的频率，并使用 Pearson χ检验进行统计学分析。单向方差分析用于检验瘦型 NAFLD 个体中阳性 SNP 与代谢参数之间的相关性。我们的结果表明，rs2279026 的 C 等位基因频率、rs2279028 的 G 等位基因、rs780093 的 C 等位基因和 rs1260326 的 C 等位基因频率在肥胖型 NAFLD 中高于瘦型 NAFLD（P<0.05）。此外，我们还观察到 FTO 基因中 rs1421085 的 CC、rs3751812 的 TT、rs8050136 的 AA 和 rs9939609 的 AA 基因型与低密度脂蛋白水平之间存在关联（P<0.05）。总之，我们的研究结果为中国老年瘦型个体中 NAFLD 的流行率、遗传特征和代谢特征提供了独特的视角。这是第一项在瘦型个体队列中研究 FTO、TFAP2B 和 GCKR 基因中遗传变异与 NAFLD 之间关系的研究。
