Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Postepu 36a, 05-552, Jastrzebiec, Poland.
Chair of Livestock Biotechnology, Technische Universität München, Liesel-Beckmann str. 1, 85354, Freising, Germany.
J Appl Genet. 2019 Nov;60(3-4):401-404. doi: 10.1007/s13353-019-00519-6. Epub 2019 Aug 30.
We previously showed that deregulation of PEG3 domain genes is associated with intrauterine growth restriction (IUGR) in cattle. Here, we carried out genome-wide DNA methylation analysis of foetal placenta in two IUGR and wild-type samples and identified a differentially methylated region (DMR) in intron 2 of MIMT1. Pyrosequencing on a larger sample size (n = 20) confirmed significantly lower (P < 0.001) MIMT1-DMR methylation in foetal and maternal placenta of IUGR than wild-type conceptuses. Our study demonstrates that small-scale whole-genome bisulphite sequencing can be used to identify epigenetic regulatory elements in a defined genome locus.
我们之前的研究表明 PEG3 结构域基因的失调与牛的宫内生长受限（IUGR）有关。在这里，我们对两个 IUGR 和野生型样本的胎儿胎盘进行了全基因组 DNA 甲基化分析，在 MIMT1 的内含子 2 中鉴定出一个差异甲基化区域（DMR）。在更大的样本量（n=20）上进行焦磷酸测序，确认 IUGR 胎儿和母体胎盘的 MIMT1-DMR 甲基化水平明显低于野生型胚胎（P<0.001）。我们的研究表明，小规模全基因组亚硫酸氢盐测序可用于鉴定特定基因组位点的表观遗传调控元件。
