Li Shun, Pausch Hubert, Venhoranta Heli, Adamowicz Krzysztof, Andersson Magnus, Zwierzchowski Lech, Kind Alexander, Schnieke Angelika, Flisikowski Krzysztof
Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
Lehrstuhl für Tierzucht, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
Anim Genet. 2016 Feb;47(1):106-9. doi: 10.1111/age.12373. Epub 2015 Nov 5.
We used a genetic (MIMT1(Del)) model of intrauterine growth restriction to investigate dysregulation of PEG3 domain gene expression in bovine foetal and maternal placenta. ZIM2, APEG3 and PEG3 expressions were similarly reduced in MIMT1(Del/) (WT) foetal placenta, suggesting coordinated regulation. Methylation of DNA CpG sites associated with these genes showed no differences, but differences in the levels of MIMT1 RNA methylation at three CpG sites were found in foetal placenta. Our data are consistent with the presence of a bidirectional promoter 5' of MIMT1 and suggest a regulatory role for the MIMT1 non-coding transcript. PEG3 domain expression on the maternal placenta side was not affected by the foetal mutation.
我们使用了子宫内生长受限的遗传（MIMT1(Del)）模型，来研究牛胎儿和母体胎盘中PEG3结构域基因表达的失调情况。在MIMT1(Del/)(WT)胎儿胎盘中，ZIM2、APEG3和PEG3的表达同样降低，表明存在协同调控。与这些基因相关的DNA CpG位点的甲基化没有差异，但在胎儿胎盘中发现了三个CpG位点处MIMT1 RNA甲基化水平的差异。我们的数据与MIMT1 5'端存在双向启动子一致，并表明MIMT1非编码转录本具有调控作用。胎儿突变并未影响母体胎盘侧的PEG3结构域表达。
