College of Bioscience, Fukui Prefectural University, Eiheiji, Japan.
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA.
Adv Exp Med Biol. 2019;1155:523-529. doi: 10.1007/978-981-13-8023-5_48.
Mammalian tissues, especially the heart, contain high concentrations of taurine, a beta-amino acid that possesses a variety of physiological functions. While it is well known that taurine reacts with several metabolites, such as bile acids and fatty acids, taurine-conjugated metabolites in the heart have not been specifically studied. Recently, we performed Liquid chromatography-mass spectrometry- (LC-MS-) based metabolome analysis, comparing metabolome profiles of hearts from taurine transporter knockout (TauTKO) mice and wild-type mice to identify differences in taurine-conjugated metabolite content of the two phenotypes. Comparison of the metabolite profiles revealed taurine-containing dipeptides, such as glutamyltaurine, which are present in wild-type but not in TauTKO hearts. These data suggest that taurine functions not only as a free osmolyte but also as a conjugated metabolite within the heart.
哺乳动物组织，特别是心脏，含有高浓度的牛磺酸，一种β-氨基酸，具有多种生理功能。虽然众所周知牛磺酸与几种代谢物（如胆汁酸和脂肪酸）反应，但心脏中的牛磺酸缀合代谢物尚未被专门研究。最近，我们进行了基于液相色谱-质谱（LC-MS）的代谢组学分析，比较了牛磺酸转运蛋白敲除（TauTKO）小鼠和野生型小鼠心脏的代谢组学图谱，以确定两种表型中牛磺酸缀合代谢物含量的差异。代谢物图谱的比较显示出牛磺酸含有二肽，如谷氨酸牛磺酸，存在于野生型心脏中，但不存在于 TauTKO 心脏中。这些数据表明，牛磺酸不仅作为游离渗透剂，而且作为共轭代谢物在心脏中发挥作用。
