育珠田螺提取物对叔丁基过氧化物诱导的氧化应激的抗氧化活性。

Antioxidant Activities of Viviparus Contectus Extract Against Tert-Butylhydroperoxide-Induced Oxidative Stress.

机构信息

BK21plus Glocal Education Program of Nutraceuticals Development, Konkuk University, Chungju, Republic of Korea.

Medicinal Biological Resources Research Institute, Konkuk University, Chungju, Republic of Korea.

出版信息

Adv Exp Med Biol. 2019;1155:611-626. doi: 10.1007/978-981-13-8023-5_55.

DOI:10.1007/978-981-13-8023-5_55

PMID:31468435

Abstract

In this study, the antioxidant properties of Viviparus contectus (V. contectus) extract were evaluated for various radical scavenging activities, ferric reducing antioxidant power (FRAP), ABTS radical scavenging activity and oxygen radical absorbance capacity (ORAC). In addition, inhibition effect of the V. contectus extract against DNA scission induced by hydroxyl radical was measured. We also studied the protective effect of V. contectus extract against oxidative damage through measurements of intracellular reactive oxygen species (ROS) in Chang cells and zebrafish embryo. We found that V. contectus extract contains strong radical scavenging activities and antioxidant properties, which prevent tert-butylhydroperoxide (t-BHP)-induced oxidative stress, enhance cell viability, reduce ROS production, inhibit oxidative damage and improve mitochondrial function in Chang cells. Also, we determined that the V. contectus extract reduced ROS production mediated by t-BHP induced oxidative stress on zebrafish embryo.

摘要

在这项研究中，评估了圆田螺（V. contectus）提取物的抗氧化特性，包括各种自由基清除活性、铁还原抗氧化能力（FRAP）、ABTS 自由基清除活性和氧自由基吸收能力（ORAC）。此外，还测量了圆田螺提取物对羟基自由基诱导的 DNA 断裂的抑制作用。我们还通过测量 Chang 细胞和斑马鱼胚胎中的细胞内活性氧（ROS），研究了圆田螺提取物的抗氧化损伤的保护作用。我们发现圆田螺提取物含有很强的自由基清除活性和抗氧化特性，可预防叔丁基过氧化物（t-BHP）诱导的氧化应激，提高细胞活力，减少 ROS 生成，抑制氧化损伤并改善 Chang 细胞中的线粒体功能。此外，我们还确定圆田螺提取物降低了 t-BHP 诱导的氧化应激对斑马鱼胚胎中 ROS 生成的影响。