Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Republic of Korea.
Natural Product Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Republic of Korea.
Adv Exp Med Biol. 2019;1155:643-659. doi: 10.1007/978-981-13-8023-5_57.
Batillus cornutus (B. cornutus) is one of the gastropoda, which are distributed along the coast of China, Japan and South Korea and northeast area. In this study, we first identified the antioxidant effects of a B. cornutus meat (BM) enzymatic hydrolysate in HO-treated Vero cells. First of all, we prepared an Alcalase hydrolysate from BM (BMA) and revealed a high taurine content. Also, taurine rich BMA dose-dependently increased 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, reducing power and the higher oxygen radical absorbance capacity (ORAC) value. In addition, BMA significantly increased the cell viability via the down-regulation of intracellular reactive oxygen species (ROS) production, as well as the decreased formation of apoptotic bodies and sub-G DNA population in HO-treated Vero cells. Furthermore, BMA increased the expression of the anti-apoptotic molecule, Bcl-2, and decreased the expressions of Bax, p53 and cleaved PARP, all of which are pro-apoptotic molecules, in HO-treated Vero cells. Based on these results, this study suggests that BMA may be used as a potential protector on damage caused by oxidative stress.
短角滨螺（B. cornutus）是腹足纲动物之一，分布在中国沿海、日本和韩国以及东北地区。在本研究中，我们首先鉴定了短角滨螺肉酶解物（BM）对 HO 处理的 Vero 细胞的抗氧化作用。首先，我们从 BM 中制备了一种 Alcalase 水解物（BMA），并揭示了其具有高牛磺酸含量。此外，富含牛磺酸的 BMA 呈剂量依赖性地增加 2,2-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性、还原能力和更高的氧自由基吸收能力（ORAC）值。此外，BMA 通过下调细胞内活性氧（ROS）的产生，以及减少 HO 处理的 Vero 细胞中凋亡小体和亚 G1 DNA 群体的形成，显著增加了细胞活力。此外，BMA 增加了抗凋亡分子 Bcl-2 的表达，并降低了 HO 处理的 Vero 细胞中促凋亡分子 Bax、p53 和 cleaved PARP 的表达。基于这些结果，本研究表明 BMA 可能可作为氧化应激损伤的潜在保护剂。
