Lee Yeon-Ji, Kim Eun-A, Kang Nalae, Park Areumi, Heo Soo-Jin
Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea.
Department of Marine Technology & Convergence Engineering (Marine Biotechnology), University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
Foods. 2023 Oct 4;12(19):3660. doi: 10.3390/foods12193660.
, a marine gastropod mollusk commonly called sea snail, is found along the southern coast of Korea and holds considerable importance as a marine food resource, particularly on Jeju Island, Korea. Data are scarce on the antioxidant activity of hot water extracts from visceral tissue. Therefore, this study was performed to evaluate the antioxidant activities of . visceral tissue hot water extract (TVE) and the underlying mechanisms against hydrogen peroxide-induced oxidative stress in Vero cells. The amino acid composition and antioxidant effects of TVE were evaluated. Furthermore, the impact of TVE on the expression of proteins within the mitogen-activated protein kinase (MAPK) pathway is investigated. TVE showed a concentration-dependent enhancement in its scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC = 1.07 ± 0.06 mg/mL) and hydrogen peroxide (IC = 0.33 ± 0.03 mg/mL). TVE reduced intracellular reactive oxygen species (ROS) production and maintained cell viability under HO-induced oxidative stress by suppressing apoptosis in Vero cells. Additionally, TVE demonstrated regulatory effects on the MAPK and protein kinase B (Akt) signaling pathways activated by HO. In conclusion, the findings from our study propose that TVE holds potential as a bioactive component in the formulation of functional foods.
作为一种通常被称为海蜗牛的海洋腹足纲软体动物，在韩国南部海岸被发现，并且作为一种海洋食物资源具有相当重要的意义，特别是在韩国济州岛。关于其内脏组织热水提取物抗氧化活性的数据很少。因此，本研究旨在评估其内脏组织热水提取物（TVE）的抗氧化活性以及针对过氧化氢诱导的Vero细胞氧化应激的潜在机制。评估了TVE的氨基酸组成和抗氧化作用。此外，研究了TVE对丝裂原活化蛋白激酶（MAPK）途径内蛋白质表达的影响。TVE对2,2-二苯基-1-苦基肼自由基（DPPH）（IC = 1.07±0.06 mg/mL）和过氧化氢（IC = 0.33±0.03 mg/mL）的清除活性呈现浓度依赖性增强。TVE通过抑制Vero细胞凋亡减少细胞内活性氧（ROS）产生并在过氧化氢诱导的氧化应激下维持细胞活力。此外，TVE对由过氧化氢激活的MAPK和蛋白激酶B（Akt）信号通路具有调节作用。总之，我们研究的结果表明TVE作为功能性食品配方中的生物活性成分具有潜力。
