三角褐指藻的电穿孔转化方案
Electroporation Transformation Protocol for Phaeodactylum tricornutum.
作者信息
Hu Hanhua, Pan Yufang
机构信息
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
出版信息
Methods Mol Biol. 2020;2050:163-167. doi: 10.1007/978-1-4939-9740-4_17.
Genetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear transformation method for the model diatom Phaeodactylum tricornutum using an electroporation system, and the maximum transformation frequency obtained is about 3 × 10 cells. The described protocol also provides some clue for developing electroporation transformation system in other eukaryotic microalgae.
遗传转化系统对于硅藻的基础生物学研究和商业开发都非常重要。在此，我们描述了一种使用电穿孔系统对模式硅藻三角褐指藻进行高效核转化的方法，获得的最大转化频率约为3×10个细胞。所描述的方案也为在其他真核微藻中开发电穿孔转化系统提供了一些线索。
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