Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California-Davis, Davis, California.
Department of Medical Microbiology and Immunology, University of California-Davis, Davis, California.
J Vet Intern Med. 2019 Sep;33(5):2082-2089. doi: 10.1111/jvim.15599. Epub 2019 Aug 29.
Point-of-care (POC) Cryptococcus antigen assays may provide veterinarians with a more rapid, patient-side diagnosis when compared with traditional laboratory-based latex agglutination tests.
To determine the sensitivity and specificity of 2 POC lateral flow cryptococcal serum antigen tests, CrAg LFA (Immy, Norman, OK) and the CryptoPS (Biosynex, Strasbourg, France) for diagnosis of cryptococcosis in dogs and cats, using the cryptococcal antigen latex agglutination system (CALAS) as the reference standard.
102 serum samples from 51 dogs and 40 cats.
Specimens were classified as CALAS-positive (n = 25) or CALAS-negative (n = 77). The sensitivity and specificity of each POC assay was calculated by comparing the results to the serologic reference standard results.
The CrAg LFA assay correctly classified 23/25 CALAS-positive specimens and 69/74 CALAS-negative specimens resulting in a sensitivity of 92.0% (confidence interval [CI], 75.0%-98.6%) and specificity of 93.2% (CI, 85.1%-97.1%). The CryptoPS assay correctly classified 8/10 tested CALAS-positive specimens and 56/59 tested CALAS-negative specimens resulting in a sensitivity of 80.0% (CI, 49.0%-96.5%) and specificity of 94.9% (CI, 86.1%-98.6%).
The POC assays appear to be a sensitive and specific alternative to the traditional CALAS assay with more rapid turnaround times, which may result in earlier diagnosis and treatment.
与传统基于实验室的乳胶凝集试验相比，即时检测（POC） cryptococcus 抗原检测可为兽医提供更快速、更贴近患者的诊断。
确定 2 种 POC 侧向流动 cryptococcal 血清抗原检测，即 CrAg LFA（来自 Immy，俄克拉荷马州诺曼）和 CryptoPS（来自 Biosynex，斯特拉斯堡，法国）在诊断犬猫 cryptococcosis 方面的敏感性和特异性，以 cryptococcal 抗原乳胶凝集系统（CALAS）为参考标准。
51 只犬和 40 只猫的 102 份血清样本。
将标本分为 CALAS 阳性（n = 25）或 CALAS 阴性（n = 77）。通过将结果与血清学参考标准结果进行比较，计算每种 POC 检测的敏感性和特异性。
CrAg LFA 检测正确分类了 25 个 CALAS 阳性标本中的 23 个和 74 个 CALAS 阴性标本中的 69 个，其敏感性为 92.0%（置信区间[CI]，75.0%-98.6%），特异性为 93.2%（CI，85.1%-97.1%）。CryptoPS 检测正确分类了 10 个测试的 CALAS 阳性标本中的 8 个和 59 个测试的 CALAS 阴性标本中的 56 个，其敏感性为 80.0%（CI，49.0%-96.5%），特异性为 94.9%（CI，86.1%-98.6%）。
与传统的 CALAS 检测相比，POC 检测似乎具有较高的敏感性和特异性，并且具有更短的周转时间，这可能导致更早的诊断和治疗。
