Translational Mycology Unit, Laboratory of Clinical Infectious Diseases, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Division of Infectious Diseases, Department of Medicine, F. Hèbert School of Medicine, Uniformed Services University of the Health Sciences (USUHS), Bethesda, Maryland, USA
Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
J Clin Microbiol. 2014 Dec;52(12):4356-8. doi: 10.1128/JCM.02017-14. Epub 2014 Sep 24.
We compared paired enzyme immunoassay (EIA) and latex agglutination (LA) assay results with 185 blood and 164 cerebrospinal fluid (CSF) samples from 44 and 33 non-HIV cryptococcosis patients, respectively. The LA assay cutoff of 1:256 in the blood and 1:32 in the CSF was most highly predictive of a positive EIA result. The EIA missed 18.4% detected by the LA assay in the blood samples and 7.8% detected by the LA assay in the CSF samples. We note here the improved sensitivity of the LA assay over the EIA in non-HIV patients.
我们比较了配对酶免疫分析（EIA）和乳胶凝集（LA）检测结果，分别用 44 名非 HIV 隐球菌病患者的 185 份血液和 164 份脑脊液（CSF）样本，以及 33 名非 HIV 隐球菌病患者的 185 份血液和 164 份脑脊液（CSF）样本。LA 检测在血液中的截断值为 1:256，在 CSF 中的截断值为 1:32，与阳性 EIA 结果最具预测性。EIA 检测漏检了 LA 检测在血液样本中检测到的 18.4%和在 CSF 样本中检测到的 7.8%。我们注意到，LA 检测在非 HIV 患者中的敏感性优于 EIA。
