College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
State Key Lab of Petroleum Pollution Control, CNPC Research Institute of Safety & Environmental Technology, Beijing 102206, China.
J Environ Sci (China). 2019 Nov;85:107-118. doi: 10.1016/j.jes.2019.05.013. Epub 2019 May 21.
Biochar (BC) and rhamnolipid (RL) is used in bioremediation of petroleum hydrocarbons, however, the combined effect of BC and RL in phytoremediation has not been studied until now. In this paper, the phytoremediation of petroleum hydrocarbon-contaminated soil using novel plant Spartina anglica was enhanced by the combination of biochar (BC) and rhamnolipid (RL). Samples of petroleum-contaminated soil (10, 30 and 50 g/kg) were amended by BC, BC+ RL and rhamnolipid modified biochar (RMB), respectively. After 60 day's cultivation, the removal rate of total petroleum hydrocarbons (TPHs) for unplanted soil (UP), planted soil (P), planted soil with BC addition (P-BC), planted soil with BC and RL addition (P-BC + RL) and planted soil with addition of RMB (P-RMB) were 8.6%, 19.1%, 27.7%, 32.4% and 35.1% in soil with TPHs concentration of 30 g/kg, respectively. Compared with UP, the plantation of Spartina anglica significantly decreased the concentration of C and tricyclic PAHs. Furthermore, the application of BC and RMB alleviated the toxicity of petroleum hydrocarbons to Spartina anglica via improving plant growth with increasing plant height, root vitality and total chlorophyll content. High-throughput sequencing result indicated that rhizosphere microbial community of Spartina anglica was regulated by the application of BC and RMB, with increase of bacteria and plant mycorrhizal symbiotic fungus in biochar and RMB amended soil.
生物炭（BC）和鼠李糖脂（RL）被用于石油烃的生物修复，然而，直到现在，BC 和 RL 在植物修复中的联合作用还没有被研究过。在本文中，通过将生物炭（BC）和鼠李糖脂（RL）与新型植物互花米草（ Spartina anglica ）相结合，增强了受石油烃污染土壤的植物修复。将生物炭（BC）、BC+ RL 和改性生物炭（RMB）分别添加到浓度为 10、30 和 50 g/kg 的石油污染土壤中。经过 60 天的培养，未种植土壤（UP）、种植土壤（P）、添加 BC 的种植土壤（P-BC）、添加 BC 和 RL 的种植土壤（P-BC+ RL）和添加 RMB 的种植土壤（P-RMB）中总石油烃（TPHs）的去除率分别为 8.6%、19.1%、27.7%、32.4%和 35.1%，土壤中 TPHs 浓度为 30 g/kg。与 UP 相比，互花米草的种植显著降低了 C 和三环多环芳烃的浓度。此外，BC 和 RMB 的应用通过提高植物高度、根系活力和总叶绿素含量来改善植物生长，从而减轻了石油烃对互花米草的毒性。高通量测序结果表明，BC 和 RMB 的应用调节了互花米草根际微生物群落，生物炭和 RMB 处理土壤中细菌和植物菌根共生真菌的数量增加。
