Bégué J M, Baffet G, Campion J P, Guillouzo A
Unité de Recherches Hépatologiques U 49, INSERM, Hôpital de Pontchaillou, Rennes, France.
Biol Cell. 1988;63(3):327-33. doi: 10.1111/j.1768-322x.1988.tb00756.x.
The acute hepatotoxicity induced by aflatoxin B1 (AFB1) and the potential protective effect of (+)-cyanidanol-3 (Catergen) were evaluated in both human and rat hepatocytes in primary cultures. AFB1-induced acute toxicity was visualized by light microscope observation and quantified by measurement of lactic dehydrogenase activity in the medium. Human hepatocytes were susceptible to AFB1-induced cytotoxicity but no evident relationship between the concentration of mycotoxin and the extent of cellular damage was established. (+)-Cyanidanol-3 was not toxic at concentrations up to 2 x 10(-3)M, but no obvious protective effect from AFB1-induced injury was evidenced in human cells. By contrast, rat hepatocytes responded in a dose-related manner to AFB1. (+)-Cyanidanol was toxic at 10(-3)M, but even at this concentration exerted a strong protective effect against AFB1-induced cytotoxicity. Such species differences suggest the existence of metabolic differences in both AFB1 and (+)-cyanidanol-3 activating and deactivating mechanisms.
在原代培养的人和大鼠肝细胞中评估了黄曲霉毒素B1（AFB1）诱导的急性肝毒性以及（+）-氰定醇-3（Catergen）的潜在保护作用。通过光学显微镜观察可视化AFB1诱导的急性毒性，并通过测量培养基中的乳酸脱氢酶活性进行定量。人肝细胞对AFB1诱导的细胞毒性敏感，但未确定霉菌毒素浓度与细胞损伤程度之间的明显关系。（+）-氰定醇-3在浓度高达2×10⁻³M时无毒，但在人细胞中未证明对AFB1诱导的损伤有明显保护作用。相比之下，大鼠肝细胞对AFB1呈剂量相关反应。（+）-氰定醇在10⁻³M时有毒，但即使在此浓度下也对AFB1诱导的细胞毒性发挥了强大的保护作用。这种物种差异表明在AFB1和（+）-氰定醇-3的激活和失活机制中存在代谢差异。
