通过直接接种BACTEC NAP小瓶对痰中结核分枝杆菌进行鉴定。

Identification of Mycobacterium tuberculosis in sputum by direct inoculation of the BACTEC NAP vial.

作者信息

Stager C E, Davis J R, Siddiqi S H

机构信息

Jefferson Davis Hospital, Department of Pathology, Baylor College of Medicine, Houston, Texas.

出版信息

Diagn Microbiol Infect Dis. 1988 Jun;10(2):67-73. doi: 10.1016/0732-8893(88)90042-9.

DOI:10.1016/0732-8893(88)90042-9

PMID:3147161

Abstract

The Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, and M. africanum) can be differentiated from mycobacteria other than M. tuberculosis (MOTT bacilli) with the BACTEC NAP test (Johnston Laboratories, Becton, Dickinson & Co., Towson, MD), by selectively inhibiting their growth with p-nitro-alpha-acetyl-amino-beta-hydroxypropriophenone (NAP). The BACTEC NAP test is recommended for isolated mycobacterial cultures. In this report, a direct NAP test is performed by immediate inoculation of BACTEC NAP vials with processed sputum specimens that stain acid-fast positive. Seventy-six of 80 M. tuberculosis were correctly identified and all of the MOTT bacilli (nine M. avium complex and one M. kanasii) were correctly classified. The average time required for identification of M. tuberculosis with the direct BACTEC NAP test described here is more convenient than the recommended indirect test, and it is an accurate and rapid method to differentiate the M. tuberculosis complex from MOTT bacilli.

摘要

结核分枝杆菌复合群（结核分枝杆菌、牛分枝杆菌和非洲分枝杆菌）可通过BACTEC NAP试验（约翰斯顿实验室，贝克顿·迪金森公司，马里兰州陶森）与非结核分枝杆菌（非结核分枝杆菌）相鉴别，该试验通过对硝基-α-乙酰氨基-β-羟基苯丙酮（NAP）选择性抑制它们的生长来实现。BACTEC NAP试验推荐用于分离的分枝杆菌培养物。在本报告中，通过将抗酸染色阳性的处理后痰液标本立即接种到BACTEC NAP小瓶中进行直接NAP试验。80株结核分枝杆菌中有76株被正确鉴定，所有非结核分枝杆菌（9株鸟分枝杆菌复合群和1株堪萨斯分枝杆菌）均被正确分类。此处所述的直接BACTEC NAP试验鉴定结核分枝杆菌所需的平均时间比推荐的间接试验更方便，且是一种区分结核分枝杆菌复合群与非结核分枝杆菌的准确、快速方法。