Anargyros P, Astill D S, Lim I S
Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J Clin Microbiol. 1990 Jun;28(6):1288-91. doi: 10.1128/jcm.28.6.1288-1291.1990.
A 4-month trial involving 2,563 routine clinical specimens was conducted to compare the improved BACTEC TB system (12B medium) with the conventional Lowenstein-Jensen (LJ) media for the isolation, identification, and susceptibility testing of mycobacteria. One hundred sixty-two mycobacterial isolates were recovered, 147 (91%) with BACTEC and 118 (73%) with LJ media. Of these, 62 were Mycobacterium tuberculosis complex strains, 59 (95%) of which were isolated with BACTEC and 54 (87%) of which were isolated with LJ media. Of the remaining 100 isolates, which were mycobacteria other than tuberculosis (MOTT), BACTEC and LJ media detected 88 and 64%, respectively. The contamination rate was significantly higher in BACTEC (5%) than in LJ media (3.3%). The mean isolation time for M. tuberculosis complex with BACTEC was 15.5 days, compared with 25.6 days with LJ. For MOTT, the mean isolation times were 5.8 and 21.4 days, respectively. Identification of 32 M. tuberculosis complex isolates and 38 isolates of MOTT by the BACTEC NAP (p-nitro-alpha-acetylamino-beta-hydroxypropiophenone) inhibition test gave 100% agreement with conventional biochemical identifications. The results of susceptibility testing of 18 M. tuberculosis complex isolates with BACTEC agreed completely with those obtained by the resistance ratio method.
进行了一项为期4个月的试验，涉及2563份常规临床标本，以比较改良的BACTEC结核系统（12B培养基）与传统的罗氏（LJ）培养基在分枝杆菌分离、鉴定及药敏试验方面的效果。共分离出162株分枝杆菌，其中使用BACTEC培养基分离出147株（91%），使用LJ培养基分离出118株（73%）。其中，62株为结核分枝杆菌复合群菌株，使用BACTEC培养基分离出59株（95%），使用LJ培养基分离出54株（87%）。其余100株分离株为非结核分枝杆菌（MOTT），BACTEC培养基和LJ培养基的检出率分别为88%和64%。BACTEC培养基的污染率（5%）显著高于LJ培养基（3.3%）。使用BACTEC培养基分离结核分枝杆菌复合群的平均时间为15.5天，而使用LJ培养基为25.6天。对于MOTT，平均分离时间分别为5.8天和21.4天。通过BACTEC NAP（对硝基-α-乙酰氨基-β-羟基苯丙酮）抑制试验对32株结核分枝杆菌复合群分离株和38株MOTT分离株进行鉴定，结果与传统生化鉴定完全一致。对18株结核分枝杆菌复合群分离株进行BACTEC药敏试验的结果与耐药率法获得的结果完全一致。
