Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA.
Nat Methods. 2013 Sep;10(9):873-5. doi: 10.1038/nmeth.2568. Epub 2013 Jul 21.
The difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome by using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins and exhibit fluorescence increases that enable protein expression to be imaged in living Escherichia coli. These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time.
通过使用基于 Spinach 的荧光传感器可以克服在活细菌细胞中成像蛋白质表达动力学的困难，Spinach 是一种 RNA，可激活小分子荧光团的荧光。这些 RNA 选择性地结合靶蛋白，并表现出荧光增加，从而可以在活的大肠杆菌中对蛋白质表达进行成像。这些传感器是实时在单个细菌中成像蛋白质表达的通用策略的关键组成部分。
