在去卵巢母羊中，对促性腺激素mRNA量进行测量，该测量是在雌二醇诱导的促黄体生成素（LH）和促卵泡生成素（FSH）排卵前样激增期间进行的。

Measurement of the amount of mRNA for gonadotropins during an estradiol-induced preovulatory-like surge of LH and FSH in ovariectomized ewes.

作者信息

Hamernik D L, Nett T M

机构信息

Department of Physiology, Colorado State University, Ft. Collins 80523.

出版信息

Domest Anim Endocrinol. 1988 Apr;5(2):129-39. doi: 10.1016/0739-7240(88)90013-6.

DOI:10.1016/0739-7240(88)90013-6

PMID:3147166

Abstract

The amount of messenger RNA (mRNA) for luteinizing hormone beta-subunit (LH beta), follicle-stimulating hormone beta-subunit (FSH beta) and alpha-subunit was measured during estradiol-17 beta (E) positive feedback in ovariectomized (OVX) ewes. During the anestrous season, OVX ewes were given an i.m. injection of E (25 micrograms: n = 5) or oil (control; n = 4) and hourly blood samples were collected for 16 hr. After blood collection, ewes were killed and anterior pituitary glands were removed for analysis of hormone and mRNA content. Preovulatory-like increases in serum concentrations of LH and FSH were measured in E-treated OVX ewes. In two E-treated OVX ewes the serum concentrations of LH and FSH were still increasing, whereas in the remaining three E-treated OVX ewes, serum concentrations of LH were on the decreasing portion of the E-induced preovulatory-like surge. Pituitary content of LH was lower (P less than .10) in E-treated OVX ewes when serum concentrations of LH were decreasing than that measured in control ewes or E-treated OVX ewes in which serum concentrations were still increasing. Pituitary content of FSH and prolactin were similar (P greater than .05) among all groups. The amount of mRNA for LH beta-subunit was similar (P greater than .05) in ewes in which serum concentrations of LH were increasing and in control ewes, but was lower (P less than .05) in ewes with decreasing levels of LH. The amount of mRNA for FSH beta-subunit was lower (P less than .05) in all E-treated OVX ewes (independent of whether serum concentrations of FSH were increasing or decreasing) than that measured in control ewes. There was no difference (P greater than .05) in the amount of mRNA for alpha-subunit among any groups. Thus, amounts of mRNA for the beta-subunits of gonadotropins are reduced, while amounts of mRNA for alpha-subunit are unchanged during estradiol positive feedback in OVX ewes.

摘要

在切除卵巢的（OVX）母羊中，于雌二醇-17β（E）正反馈期间测定了促黄体生成素β亚基（LHβ）、促卵泡生成素β亚基（FSHβ）和α亚基的信使核糖核酸（mRNA）量。在乏情季节，给切除卵巢的母羊肌肉注射E（25微克：n = 5）或油（对照；n = 4），并每小时采集一次血样，共采集16小时。采血后，处死母羊并取出垂体前叶，用于分析激素和mRNA含量。在接受E处理的切除卵巢的母羊中检测到促黄体生成素（LH）和促卵泡生成素（FSH）血清浓度出现类似排卵前的升高。在两只接受E处理的切除卵巢的母羊中，LH和FSH的血清浓度仍在升高，而在其余三只接受E处理的切除卵巢的母羊中，LH的血清浓度处于E诱导的类似排卵前激增的下降阶段。当LH血清浓度下降时，接受E处理的切除卵巢的母羊垂体中LH的含量低于（P < 0.10）对照母羊或LH血清浓度仍在升高的接受E处理的切除卵巢的母羊。所有组中促卵泡生成素（FSH）和催乳素的垂体含量相似（P > 0.05）。LHβ亚基的mRNA量在LH血清浓度升高的母羊和对照母羊中相似（P > 0.05），但在LH水平下降的母羊中较低（P < 0.05）。所有接受E处理的切除卵巢的母羊（无论FSH血清浓度是升高还是降低）中FSHβ亚基的mRNA量均低于（P < 0.05）对照母羊。任何组中α亚基的mRNA量均无差异（P > 0.05）。因此，在切除卵巢的母羊中，雌二醇正反馈期间促性腺激素β亚基的mRNA量减少，而α亚基的mRNA量不变。